Department of Biology and Center for Life in Extreme Environments, Portland State University, Portland, OR 97201, USA.
Royal Netherlands Institute for Sea Research, Department of Marine Organic Biogeochemistry, 1790 AB Den Burg, Texel, The Netherlands.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4724-4729. doi: 10.1099/ijs.0.050518-0. Epub 2013 Aug 19.
A novel moderately thermophilic, heterotrophic bacterium was isolated from a deep-sea hydrothermal vent deposit from the Mariner field along the Eastern Lau Spreading Center of the south-western Pacific Ocean. Cells were short motile rods (about 0.4×0.8 µm) that occurred singly or in pairs and were surrounded by a sheath-like membrane or 'toga'. The cells grew between 45 and 65 °C (optimum 57-60 °C) and at pH 4.1-6.0 (optimum pH 5.5-5.7) and grew optimally at 3 % (w/v) NaCl. The isolate grew on a range of carbon and proteinaceous substrates and reduced sulfur. The G+C content of the DNA was about 45 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the new isolate as a deeply diverging lineage within the order Thermotogales. Based on the physiological, morphological and phylogenetic data, the isolate represents a novel species of a new genus with the proposed name Mesoaciditoga lauensis gen. nov., sp. nov. The type strain of Mesoaciditoga lauensis is cd-1655R(T) ( = DSM 25116(T) = OCM 1212(T)).
从西南太平洋东劳扩张中心马琳海域深海热液喷口沉积物中分离到一株新型中度嗜热异养细菌。细胞为短的运动杆状（约 0.4×0.8 µm），单个或成对存在，周围有鞘状膜或“长袍”。细胞在 45-65°C（最佳 57-60°C）和 pH4.1-6.0（最佳 pH5.5-5.7）下生长，在 3%（w/v）NaCl 下生长最佳。该分离株可在多种碳源和蛋白质底物以及还原硫上生长。DNA 的 G+C 含量约为 45 mol%。16S rRNA 基因序列的系统发育分析将新分离株置于热袍菌目内的一个深度分支谱系中。基于生理、形态和系统发育数据，该分离株代表了一个新属的新型物种，命名为 Lauensis 中温嗜酸菌（Mesoaciditoga lauensis），新种。Lauensis 中温嗜酸菌的模式菌株为 cd-1655R（T）（=DSM 25116（T）=OCM 1212（T））。
