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来自东太平洋热液沉积物的模式菌株SLHLJ1的全基因组序列及全基因组系统发育分析

Complete genome sequence and whole-genome phylogeny of type strain SLHLJ1 from an East Pacific hydrothermal sediment.

作者信息

Jiang Lijing, L'Haridon Stéphane, Jebbar Mohamed, Xu Hongxiu, Alain Karine, Shao Zongze

机构信息

Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen, 361005 China ; State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005 China ; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005 China.

Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France ; CNRS, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France ; Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Technopôle Brest-Iroise, BP70, 29 280 Plouzané, France.

出版信息

Stand Genomic Sci. 2017 Jan 5;12:3. doi: 10.1186/s40793-016-0214-2. eCollection 2017.

DOI:10.1186/s40793-016-0214-2
PMID:28074121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5217533/
Abstract

strain SLHLJ1 is a thermophilic chemoorganoheterotrophic bacterium isolated from a deep-sea hydrothermal sediment. It belongs to the physiologically homogeneous family. Here, we describe the phenotypic features of together with its genome sequence and annotation. The chromosome has 2,169,170 bp, organized in one contig. A total of 1897 candidate protein-encoding genes and 177 RNA genes were identified. The 16S rRNA gene sequence of this strain is distantly related to sequences of some relatives classified in the same genus ( 7.02% and 7.83%), with dissimilarity percentages close to the threshold generally described for genus delineation. Nevertheless, the percentage of conserved proteins (POCP), which is much higher than 50% (around 70%), together with phenotypic features of the isolates, confirm the affiliation all species described so far to the same genus.

摘要

菌株SLHLJ1是从深海热液沉积物中分离出的嗜热化能有机异养细菌。它属于生理上同质的家族。在此,我们描述了其表型特征以及基因组序列和注释。该染色体有2,169,170 bp,由一个重叠群组成。共鉴定出1897个候选蛋白质编码基因和177个RNA基因。该菌株的16S rRNA基因序列与同一属中一些相关菌株的序列关系较远(分别为7.02%和7.83%),差异百分比接近通常用于界定属的阈值。然而,保守蛋白百分比(POCP)远高于50%(约70%),连同分离株的表型特征,证实了迄今为止所描述的所有物种都属于同一属。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/37e31dba7fcf/40793_2016_214_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/4e16bf7f50d2/40793_2016_214_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/51a5df46a532/40793_2016_214_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/b089ec0f9bf6/40793_2016_214_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/37e31dba7fcf/40793_2016_214_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/4e16bf7f50d2/40793_2016_214_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/51a5df46a532/40793_2016_214_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/b089ec0f9bf6/40793_2016_214_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d402/5217533/37e31dba7fcf/40793_2016_214_Fig4_HTML.jpg

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