Department of Biotechnology, PRIST University, Thanjavur, Tamilnadu, India.
Saudi J Biol Sci. 2011 Apr;18(2):181-7. doi: 10.1016/j.sjbs.2010.11.001. Epub 2010 Nov 11.
The intention of this investigation was to evaluate the free radical scavenging activity and erythrocyte protective activity of ethanolic extract of Crinum asiaticum (L) and lycorine. The ethanolic extract of C. asiaticum (L) and lycorine were found to have different levels of antioxidant properties in the test models. Both ethanolic extract of C. asiaticum (L) (0.5-2.5 mg/ml) and lycorine (0.010 mg-0.050 mg/ml) increases the percentage of lipid peroxidation inhibition (26.25 ± 0.23% and 19.25 ± 0.23%) and enhances the free radical scavenging activity (20.92 ± 0.22% and 20.52 ± 0.22%), scavenging of hydrogen peroxide (25.67 ± 0.17% and 23.07 ± 0.3%) superoxide anion scavenging activity (27.69 ± 0.16% and 16.09 ± 0.7%) at concentration of 2.5 and 0.050 mg of C. asiaticum (L) and lycorine, respectively. But compared with tocopherol (P < 0.05) less activity was observed by C. asiaticum (L) and lycorine. The ethanolic extract of C. asiaticum (L) and lycorine were normalized to reduce the level of glutathione and also to sustain the status of protein in erythrocytes during the peroxyl radical [2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)] induced oxidative damage in ex vivo model. The present results of the investigations demonstrated that protective nature of the C. asiaticum (L) and lycorine will be considered as a significant natural antioxidant source.
本研究旨在评估亚洲百合（L）乙醇提取物和石蒜碱的自由基清除活性和红细胞保护活性。在测试模型中，发现亚洲百合（L）乙醇提取物（0.5-2.5mg/ml）和石蒜碱具有不同水平的抗氧化特性。亚洲百合（L）乙醇提取物（0.5-2.5mg/ml）和石蒜碱（0.010mg-0.050mg/ml）均能提高脂质过氧化抑制率（26.25±0.23%和 19.25±0.23%），增强自由基清除活性（20.92±0.22%和 20.52±0.22%），清除过氧化氢（25.67±0.17%和 23.07±0.3%）超氧阴离子清除活性（27.69±0.16%和 16.09±0.7%），浓度分别为 2.5 和 0.050mg 的亚洲百合（L）和石蒜碱。但与生育酚（P<0.05）相比，亚洲百合（L）和石蒜碱的活性较低。亚洲百合（L）乙醇提取物和石蒜碱能使谷胱甘肽水平正常化，并在过氧自由基[2,2-偶氮双（2-脒基丙烷）二盐酸盐（AAPH）]诱导的体外模型氧化损伤中维持红细胞中蛋白质的状态。本研究结果表明，亚洲百合（L）和石蒜碱的保护特性将被视为一种重要的天然抗氧化剂来源。
