Shen Hongwei, Zhang Xibin, Gong Zhiwei, Wang Yanan, Yu Xue, Yang Xiaobing, Zhao Zongbao K
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Rd., Dalian, 116023, People's Republic of China.
State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang, 437000, People's Republic of China.
Appl Microbiol Biotechnol. 2017 May;101(9):3801-3809. doi: 10.1007/s00253-017-8157-0. Epub 2017 Feb 6.
Lipid production by the red yeast Rhodosporidium toruloides was explored under nutrient limitation. To determine the compositional profiles of R. toruloides cells, samples were prepared using a continuous cultivation process under nutrient limitation and analyzed via several methods, including Fourier transform infrared spectroscopy and elemental analysis. Under nitrogen limitation, as the dilution rate increased, the cellular lipid content decreased but the carbohydrate and protein contents increased. Under carbon limitation, the cellular lipid, protein, and carbohydrate contents remained relatively constant at the different dilution rates. Moreover, the cellular elemental composition was essentially identical under nitrogen and carbon limitation at a high dilution rate of 0.20 h. We also analyzed the consumed carbon to nitrogen (C/N) under different nutrition conditions. The results indicated that the consumed C/N had a major influence on cell metabolism and product formation, which contributed to our understanding of the physiological characteristics of R. toruloides.
在营养限制条件下探究了红酵母（Rhodosporidium toruloides）的脂质生产情况。为了确定红酵母细胞的组成概况，使用营养限制下的连续培养过程制备样品，并通过多种方法进行分析，包括傅里叶变换红外光谱法和元素分析。在氮限制条件下，随着稀释率的增加，细胞脂质含量降低，但碳水化合物和蛋白质含量增加。在碳限制条件下，细胞脂质、蛋白质和碳水化合物含量在不同稀释率下保持相对恒定。此外，在0.20 h的高稀释率下，氮限制和碳限制条件下细胞的元素组成基本相同。我们还分析了不同营养条件下消耗的碳氮比（C/N）。结果表明，消耗的C/N对细胞代谢和产物形成有重大影响，这有助于我们了解红酵母的生理特性。
