Gag 依赖性 HIV-1 RNA 在极化 T 细胞的纤毛膜附近的富集。
Gag-dependent enrichment of HIV-1 RNA near the uropod membrane of polarized T cells.
机构信息
Viral Recombination Section.
出版信息
J Virol. 2013 Nov;87(21):11912-5. doi: 10.1128/JVI.01680-13. Epub 2013 Aug 21.
Abstract
The enrichment of HIV-1 macromolecules at the uropod of polarized T cells can significantly promote virus assembly and cell-mediated infection. Using live-cell fluorescence microscopy, we demonstrate that full-length HIV-1 RNA is enriched at the uropod membrane; furthermore, the presence of HIV-1 Gag containing a functional nucleocapsid domain is necessary for this HIV-1 RNA enrichment. The results from these studies provide novel insights into the mechanism of HIV-1 replication in polarized T cells.
摘要
HIV-1 大分子在极化 T 细胞的尾状突起处的富集可以显著促进病毒组装和细胞介导的感染。通过使用活细胞荧光显微镜，我们证明全长 HIV-1 RNA 富集在尾状突起膜上；此外，含有功能性核衣壳结构域的 HIV-1 Gag 的存在对于这种 HIV-1 RNA 富集是必需的。这些研究的结果为 HIV-1 在极化 T 细胞中的复制机制提供了新的见解。
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