Department of Neurological Surgery, University of Miami, Miami, FL, USA.
J Neurosci. 2013 Aug 21;33(34):13882-7. doi: 10.1523/JNEUROSCI.2524-13.2013.
Injury to the CNS leads to formation of scar tissue, which is important in sealing the lesion and inhibiting axon regeneration. The fibrotic scar that comprises a dense extracellular matrix is thought to originate from meningeal cells surrounding the CNS. However, using transgenic mice, we demonstrate that perivascular collagen1α1 cells are the main source of the cellular composition of the fibrotic scar after contusive spinal cord injury in which the dura remains intact. Using genetic lineage tracing, light sheet fluorescent microscopy, and antigenic profiling, we identify collagen1α1 cells as perivascular fibroblasts that are distinct from pericytes. Our results identify collagen1α1 cells as a novel source of the fibrotic scar after spinal cord injury and shift the focus from the meninges to the vasculature during scar formation.
中枢神经系统损伤会导致疤痕组织的形成，这对于封闭损伤部位和抑制轴突再生非常重要。由密集细胞外基质组成的纤维性瘢痕被认为起源于围绕中枢神经系统的脑膜细胞。然而，我们利用转基因小鼠证明，在硬脑膜完整的挫伤性脊髓损伤后，血管周胶原 1α1 细胞是纤维性瘢痕细胞成分的主要来源。通过遗传谱系追踪、光片荧光显微镜和抗原分析，我们确定胶原 1α1 细胞是血管周成纤维细胞，与周细胞不同。我们的结果确定了胶原 1α1 细胞是脊髓损伤后纤维性瘢痕的一个新来源，并将瘢痕形成过程中的焦点从脑膜转移到血管。
