通过超微镜对血管进行三维重建。
3D-reconstruction of blood vessels by ultramicroscopy.
机构信息
Vienna University of Technology; Institute of Solid State Electronics; Dept. of Bioelectronics; Vienna, Austria; Center for Brain Research; Medical University of Vienna; Vienna, Austria; University Oldenburg; Dept. of Neurobiology; Oldenburg, Germany.
出版信息
Organogenesis. 2009 Oct;5(4):227-30. doi: 10.4161/org.5.4.10403.
Abstract
As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with microm resolution. Thus, it can be applied to anatomical studies of numerous biological and medical specimens. We reconstructed the three-dimensional architecture of tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology of filigree branches of the microvasculature was visualized. Since tumors require an extensive growth of blood vessels to survive, this novel approach may open up new vistas in neurobiology and histology, particularly in cancer research.
摘要
最近的研究表明，超微镜（UM）可以实现具有微米分辨率的大型微观结构的 3D 可视化。因此，它可以应用于许多生物和医学标本的解剖学研究。我们通过 UM 对整个小鼠器官中的番茄凝集素（Lycopersicon esculentum）染色的血管网络进行了三维重建。可视化了微脉管系统的细丝状分支的拓扑结构。由于肿瘤需要广泛的血管生长才能存活，因此这种新方法可能会为神经生物学和组织学开辟新的视野，特别是在癌症研究方面。
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