A.N. Bach Institute of Biochemistry Moscow, Russia.
Front Genet. 2013 Aug 7;4:144. doi: 10.3389/fgene.2013.00144. eCollection 2013.
Tissue renewal is a well-known phenomenon by which old and dying-off cells of various tissues of the body are replaced by progeny of local or circulating stem cells (SCs). An interesting question is whether donor SCs are capable to prolong the lifespan of an aging organism by tissue renewal. In this work, we investigated the possible use of bone marrow (BM) SC for lifespan extension. To this purpose, chimeric C57BL/6 mice were created by transplanting BM from young 1.5-month-old donors to 21.5-month-old recipients. Transplantation was carried out by means of a recently developed method which allowed to transplant without myeloablation up to 1.5 × 10(8) cells, that is, about 25% of the total BM cells of the mouse. As a result, the mean survival time, counting from the age of 21.5 months, the start of the experiment, was +3.6 and +5.0 (±0.1) months for the control and experimental groups, respectively, corresponding to a 39 ± 4% increase in the experimental group over the control. In earlier studies on BM transplantation, a considerably smaller quantity of donor cells (5 × 10(6)) was used, about 1% of the total own BM cells. The recipients before transplantation were exposed to a lethal (for control animals) X-ray dose which eliminated the possibility of studying the lifespan extension by this method.
组织更新是一种众所周知的现象，通过这种现象，身体各种组织的老化和凋亡细胞被局部或循环干细胞（SCs）的后代所取代。一个有趣的问题是，供体干细胞是否能够通过组织更新延长衰老生物体的寿命。在这项工作中，我们研究了骨髓（BM）干细胞用于延长寿命的可能性。为此，通过将来自 1.5 个月龄年轻供体的 BM 移植到 21.5 个月龄受体中，创建了嵌合 C57BL/6 小鼠。移植是通过最近开发的一种方法进行的，该方法允许在不进行骨髓清除的情况下移植高达 1.5×10^8 个细胞，即大约是小鼠 BM 细胞总数的 25%。结果，从 21.5 个月龄，即实验开始时计算的平均存活时间，对照组和实验组分别为+3.6 和+5.0（±0.1）个月，实验组相对于对照组的存活时间延长了 39±4%。在之前的 BM 移植研究中，使用了数量相当少的供体细胞（5×10^6），大约是自身 BM 细胞总数的 1%。在移植前，受体接受了致死（对对照动物）X 射线剂量，这排除了通过这种方法研究寿命延长的可能性。
