Food Science Department, Faculty of Veterinary Medicine, University of Liège, B43bis, Sart-Tilman, 4000 Liège, Belgium.
Int J Food Microbiol. 2013 Sep 2;166(2):256-62. doi: 10.1016/j.ijfoodmicro.2013.07.017. Epub 2013 Jul 29.
The objective of this study was to evaluate the presence of Clostridium difficile in intestinal and carcass samples collected from pigs and cattle at a single slaughterhouse. C. difficile was isolated in 1% and 9.9% of the pig and cattle intestinal contents and in 7.9% and 7% of cattle and pig carcass samples respectively. A total of 19 different PCR-ribotypes were identified, among them types 078 and 014. Seven of 19 ribotypes correlated with the PCR-ribotypes involved in human C. difficile infections in Belgium. This study confirms that animals are carriers of C. difficile at slaughter and ribotypes are identical than those in humans, and that carcass contamination occurs inside the slaughterhouse.
本研究旨在评估在单一屠宰场采集的猪和牛的肠道和尸体样本中是否存在艰难梭菌。艰难梭菌分别在 1%和 9.9%的猪和牛肠道内容物以及 7.9%和 7%的牛和猪尸体样本中被分离出来。共鉴定出 19 种不同的 PCR-核糖体分型，其中包括 078 型和 014 型。19 种核糖体分型中有 7 种与比利时涉及人类艰难梭菌感染的 PCR-核糖体分型相关。本研究证实，动物在屠宰时是艰难梭菌的携带者，且其核糖体分型与人类相同，并且在屠宰场内发生了尸体污染。
