INRA.
Appl Environ Microbiol. 2013 Nov;79(21):6626-36. doi: 10.1128/AEM.02316-13. Epub 2013 Aug 23.
Fungi compete against each other for environmental resources. These interspecific combative interactions encompass a wide range of mechanisms. In this study, we highlight the ability of the white-rot fungus Pycnoporus coccineus to quickly overgrow or replace a wide range of competitor fungi, including the gray-mold fungus Botrytis cinerea and the brown-rot fungus Coniophora puteana. To gain a better understanding of the mechanisms deployed by P. coccineus to compete against other fungi and to assess whether common pathways are used to interact with different competitors, differential gene expression in P. coccineus during cocultivation was assessed by transcriptome sequencing and confirmed by quantitative reverse transcription-PCR analysis of a set of 15 representative genes. Compared with the pure culture, 1,343 transcripts were differentially expressed in the interaction with C. puteana and 4,253 were differentially expressed in the interaction with B. cinerea, but only 197 transcripts were overexpressed in both interactions. Overall, the results suggest that a broad array of functions is necessary for P. coccineus to replace its competitors and that different responses are elicited by the two competitors, although a portion of the mechanism is common to both. However, the functions elicited by the expression of specific transcripts appear to converge toward a limited set of roles, including detoxification of secondary metabolites.
真菌之间为争夺环境资源而相互竞争。这些种间对抗性相互作用包含广泛的机制。在本研究中，我们强调了白腐菌 Pycnoporus coccineus 快速生长或取代多种竞争真菌（包括灰霉病菌 Botrytis cinerea 和褐腐菌 Coniophora puteana）的能力。为了更好地了解 P. coccineus 与其他真菌竞争所采用的机制，并评估是否使用共同途径与不同的竞争者相互作用，我们通过转录组测序评估了 P. coccineus 在共培养过程中的差异基因表达，并通过对 15 个代表性基因的定量逆转录 PCR 分析进行了验证。与纯培养相比，在与 C. puteana 的相互作用中有 1,343 个转录本差异表达，在与 B. cinerea 的相互作用中有 4,253 个转录本差异表达，但只有 197 个转录本在两种相互作用中均过表达。总的来说，这些结果表明，P. coccineus 需要广泛的功能来取代其竞争者，而且两种竞争者都会引发不同的反应，尽管部分机制是两者共有的。然而，特定转录本表达引发的功能似乎集中在有限的作用上，包括对次生代谢物的解毒。
