Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Tsurumi University, 2-1-3, Tsrumi, Tsurumi-ku, Yokohama, Kanagawa 230-8501, Japan; Department of Rheumatology and Clinical Immunology, Clinical Research Center for Rheumatology and Allergy, Sagamihara National Hospital, National Hospital Organization, 18-1, Sakura-dai, Minami-ku, Sagamihara, Kanagawa 252-0392, Japan; (c)Department of Oral and Maxillofacial Surgery, Toshiba Rinkan Hospital, 7-9-1, Kamitsuruma, Sagamihara, Kanagawa 252-0385, Japan.
Cell Immunol. 2013 Jul-Aug;284(1-2):163-71. doi: 10.1016/j.cellimm.2013.07.010. Epub 2013 Aug 2.
Nickel (Ni) can cause delayed-type hypersensitivity reactions, which are thought to be mediated by the accumulation of T cells into inflamed skin. Accumulated T cells at the developmental stages in metal allergy are poorly characterized because a suitable animal model has not been established. To investigate the accumulated T cells in allergic inflamed skin, we generated a novel murine model of Ni-induced allergy. The murine model of Ni allergy was induced by two sensitizations of Ni plus lipopolysaccharide solution into the groin followed by three challenges with Ni solution into the footpad. Here we show that a specific TCR repertoire bearing Vα14Jα18, called natural killer (NK) T cells, was expanded monoclonally in BALB/c or C57BL/6 mice. Accumulation of NKT cells was characterized as CD4(+) or CD4(-)CD8(-) T cells. These results suggested that NKT cells are major pathogenic T cells at the elicitation phase of Ni allergy.
镍 (Ni) 可引起迟发型超敏反应，据认为是由 T 细胞在炎症皮肤中的积累介导的。由于尚未建立合适的动物模型，因此对金属过敏中发育阶段的累积 T 细胞的特征描述并不完善。为了研究过敏炎症皮肤中的累积 T 细胞，我们建立了一种新型的 Ni 诱导过敏的小鼠模型。通过在腹股沟处用 Ni 加脂多糖溶液进行两次致敏，然后在足底用 Ni 溶液进行三次挑战，诱导 Ni 过敏的小鼠模型。在这里，我们显示在 BALB/c 或 C57BL/6 小鼠中，特异性 TCR 谱系 Vα14Jα18 （称为自然杀伤 (NK) T 细胞）被单克隆扩增。NKT 细胞的积累被表征为 CD4(+)或 CD4(-)CD8(-)T 细胞。这些结果表明，在 Ni 过敏的激发阶段，NKT 细胞是主要的致病性 T 细胞。
