Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi, India.
FEMS Microbiol Lett. 2013 Nov;348(2):87-96. doi: 10.1111/1574-6968.12241. Epub 2013 Sep 10.
Plasmids are and will remain important cloning vehicles for biotechnology. They have also been associated with the spread of a number of diseases and therefore are a subject of environmental concern. With the advent of sequencing technologies, the database of plasmids is increasing. It will be of immense importance to identify the various bacterial hosts in which the plasmid can replicate. The present review article describes the features that confer broad host range to the plasmids, the molecular basis of plasmid host range evolution, and applications in recombinant DNA technology and environment.
质粒是并且将继续是生物技术中重要的克隆载体。它们也与许多疾病的传播有关，因此是环境关注的一个问题。随着测序技术的出现，质粒数据库正在增加。确定质粒可以复制的各种细菌宿主将是非常重要的。本文综述了赋予质粒广泛宿主范围的特征、质粒宿主范围进化的分子基础，以及在重组 DNA 技术和环境中的应用。
