De Lange G L, De Putter C, De Wijs F L
Department of Oral Cell Biology, Academic Center for Dentistry, Amsterdam, The Netherlands.
J Biomed Mater Res. 1990 Jul;24(7):829-45. doi: 10.1002/jbm.820240704.
This article describes the histological and ultrastructural appearance of the interface created in the implantation bed, between bone tissue and implants made of dense sintered hydroxyapatite (HA). Biopsies from dog subjects included: a) loaded permucosal dental implants for tooth substitution, b) subperiosteally placed implants for alveolar bone correction, c) endosseously placed dental root implants to retain ridge form following extraction. The light and electron microscopical results show extensive bone apposition on the osseous sides of the implant surfaces. There is an intimate, direct bone contact without any visible interruption. The bone is of normal lamellar type and continuously connected with the trabecular bone. Bone has grown into the finest surface irregularities of the implant. Collagen fibers of the calcified bone matrix are observed within a distance less than 500 A from the implant surface. The thin (20-100 A) electron dense layer at the bone-implant interface resembled the lamina limitans of organic bone matrix, also seen at the inner walls of the osteocytes lacunes. Deposition of bone gives rise to a biologically stable bone-implant interface, without disturbance of the physiological bone turnover. This is seen as very favorable for desired long term fixation of implant to bone.
本文描述了在植入床中骨组织与致密烧结羟基磷灰石（HA）制成的植入物之间形成的界面的组织学和超微结构外观。取自犬类受试者的活检样本包括：a）用于牙齿替代的负载式穿龈牙种植体；b）用于牙槽骨矫正的骨膜下植入物；c）拔牙后用于保留牙槽嵴形态的骨内牙根种植体。光学显微镜和电子显微镜结果显示，植入物表面的骨侧有广泛的骨附着。存在紧密、直接的骨接触，没有任何可见的中断。骨为正常的板层类型，并与小梁骨连续相连。骨已长入植入物最细微的表面不规则处。在距植入物表面小于500埃的距离内观察到钙化骨基质的胶原纤维。骨-植入物界面处薄（20-100埃）的电子致密层类似于有机骨基质的限制板，在骨细胞腔隙的内壁也可见到。骨的沉积产生了生物稳定的骨-植入物界面，而不会干扰生理性骨转换。这对于植入物与骨的理想长期固定非常有利。
