Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Scand J Immunol. 2013 Nov;78(5):478-84. doi: 10.1111/sji.12105.
Cutaneous leishmaniasis (CL) is a self-healing skin disease which rarely for unknown reason(s) the lesion develops to a non-healing form. It seems that the initial contact of Leishmania parasites with the host innate immune system is an important step in the outcome of the disease. Recent studies suggested that toll-like receptors (TLRs) play a role in Leishmania recognition. In this study, the level of TLR2 and TLR4 was checked in patients with healing form of lesion and compared with that of patients with non-healing form of lesion caused by Leishmania major. Gene expression of TLR2 and TLR4 in peripheral blood-derived macrophages, before and after stimulation with live L. major promastigotes, was evaluated using quantitative real-time reverse transcription PCR and flow cytometry. The results showed that the mean relative gene expression and difference membrane expression of TLR2 in macrophages of patients with healing form of lesion were significantly higher than patients with non-healing form of lesion (P < 0.0001 and P = 0.0034), respectively, and the mean relative gene expression and difference in protein expression of TLR4 in macrophages of patients with healing form of lesion were significantly higher than that of patients with non-healing form of lesion (P = 0.021 and P = 0.002), respectively. The data suggested a possible role for TLR2 and TLR4 in the outcome of CL lesion. Further studies are needed to understand more about the detail role of the immune factors in leishmaniasis.
皮肤利什曼病（CL）是一种自限性皮肤病，但其病变偶尔会在不明原因的情况下发展为不愈合的形式。似乎利什曼原虫寄生虫与宿主固有免疫系统的最初接触是疾病结局的一个重要步骤。最近的研究表明， toll 样受体（TLR）在利什曼原虫的识别中起作用。在这项研究中，检查了愈合形式病变患者的 TLR2 和 TLR4 水平，并与利什曼原虫引起的非愈合形式病变患者进行了比较。使用定量实时逆转录 PCR 和流式细胞术评估了外周血来源的巨噬细胞在受到活 L. major 前鞭毛体刺激前后 TLR2 和 TLR4 的基因表达。结果表明，愈合形式病变患者巨噬细胞中 TLR2 的平均相对基因表达和差异膜表达明显高于非愈合形式病变患者（P < 0.0001 和 P = 0.0034），而愈合形式病变患者中 TLR4 的平均相对基因表达和差异蛋白表达也明显高于非愈合形式病变患者（P = 0.021 和 P = 0.002）。这些数据表明 TLR2 和 TLR4 在 CL 病变结局中可能起作用。需要进一步研究以了解更多关于免疫因素在利什曼病中的详细作用。
