Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
Eye Contact Lens. 2013 Sep;39(5):329-34. doi: 10.1097/ICL.0b013e31829fae00.
To test the feasibility of measuring the entire thickness profiles of the epithelium and contact lens (CL) in vivo, using high-speed and high-resolution spectral-domain optical coherence tomography (SD-OCT).
A custom-built, long scan depth SD-OCT was developed based on a complementary metal oxide semiconductor (CMOS) camera, and the axial resolution was approximately 5.1 μm in tissue. Five eyes of five subjects were imaged twice across the horizontal meridian before and while wearing one CL. Semiautomatic measurement was done to yield the entire thickness profiles of the epithelium, total cornea, and CL after correcting for optical distortion.
The full width and depth of the epithelium, ocular surface, and CL were clearly visualized. The epithelial thickness at the center was 51.9±3.5 μm; it remained at this thickness across the central 7 mm diameter and then increased at both temporal and nasal peripheries. The CL profile showed the thinnest point at the center with thickness of 100.3±4.9 μm. The thickness increased toward the midperiphery and then decreased at the edge.
This pilot study demonstrated the feasibility of using high-speed CMOS-based OCT to evaluate the entire thickness profiles of the epithelium and CL in vivo. Further development will be needed to extend the scanning from 2D to 3D with a robust automatic image processing ability.
测试使用高速、高分辨率谱域光学相干断层扫描（SD-OCT）活体测量上皮和接触镜（CL）全厚度轮廓的可行性。
基于互补金属氧化物半导体（CMOS）相机开发了一种定制的长扫描深度 SD-OCT，在组织中的轴向分辨率约为 5.1μm。在佩戴一个 CL 前后，对 5 名受试者的 5 只眼睛进行了两次水平子午线的成像。经过光学失真校正后，进行半自动测量，以获得上皮、整个角膜和 CL 的全厚度轮廓。
可清晰显示上皮、眼表面和 CL 的全宽度和深度。中央上皮厚度为 51.9±3.5μm；在中央 7mm 直径范围内保持不变，然后在颞侧和鼻侧周边增加。CL 轮廓显示中心最薄点，厚度为 100.3±4.9μm。厚度向中周边增加，然后在边缘减少。
这项初步研究表明，使用高速 CMOS 基 OCT 活体评估上皮和 CL 的全厚度轮廓是可行的。需要进一步开发具有强大自动图像处理能力的 2D 到 3D 扫描。
