Bao Yongzhan, Jing Cui, Shi Wanyu
College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, PR China.
Afr J Tradit Complement Altern Med. 2012 Jul 1;9(4):548-52. doi: 10.4314/ajtcam.v9i4.12. eCollection 2012.
The Chinese herbal formula consisting of Astragalus membranaceus, Epimedium brevicornum, Paeoniae Alba Radix and Radix Ophiopogonis in proper proportions were adopted in order to investigate the immunoenhancing properties of the herbal formula. Fifty ICR mice were randomly divided into 5 groups (NS- NS+Hy-L+Hy-M+Hy-H+Hy ). The mice in hydrocortisone (Hy) groups were injected with hydrocortisone i.p. to induce the immunosuppressive condition. The mice in group NS were administered with normal saline as controls. The mice in groups NS+Hy-L+Hy-M+Hy-H+Hy were administered with normal saline, low, moderate and high dose of the herbal prescription respectively by gavage for 6 days. The level of serum hemolysin, the function of antibody function cell-AFC-and CD4⁺/CD8⁺ T cell ratio were measured at the end of experiments. The results showed that the level of serum hemolysin, the function of AFC and CD4⁺/CD8⁺ T cell ratio in L+Hy-M+Hy-H+Hy groups increased significantly compared with those in NS or NS+Hy groups. These results indicate that Chinese herbal medicine prescription can enhance humoral immunity and cellular immune function of the immunosuppressive mouse.
采用由黄芪、淫羊藿、白芍和麦冬按适当比例组成的中药配方，以研究该中药配方的免疫增强特性。将50只ICR小鼠随机分为5组（NS-NS+Hy-L+Hy-M+Hy-H+Hy）。给氢化可的松（Hy）组小鼠腹腔注射氢化可的松以诱导免疫抑制状态。给NS组小鼠给予生理盐水作为对照。给NS+Hy-L+Hy-M+Hy-H+Hy组小鼠分别通过灌胃给予生理盐水、低、中、高剂量的中药方剂，持续6天。在实验结束时测量血清溶血素水平、抗体功能细胞-AFC-的功能以及CD4⁺/CD8⁺T细胞比值。结果表明，与NS组或NS+Hy组相比，L+Hy-M+Hy-H+Hy组的血清溶血素水平、AFC功能和CD4⁺/CD8⁺T细胞比值显著升高。这些结果表明，中药方剂可增强免疫抑制小鼠的体液免疫和细胞免疫功能。
