Alam Nuhu, Shim Mi Ja, Lee Min Woong, Shin Pyeong Gyun, Yoo Young Bok, Lee Tae Soo
Department of Biology, University of Incheon, Incheon 406-840, Korea.
Mycobiology. 2009 Sep;37(3):183-8. doi: 10.4489/MYCO.2009.37.3.183. Epub 2009 Sep 30.
The molecular phylogeny in nine different commercial cultivated strains of Pleurotus nebrodensis was studied based on their internal transcribed spacer (ITS) region and RAPD. In the sequence of ITS region of selected strains, it was revealed that the total length ranged from 592 to 614 bp. The size of ITS1 and ITS2 regions varied among the strains from 219 to 228 bp and 211 to 229 bp, respectively. The sequence of ITS2 was more variable than ITS1 and the region of 5.8S sequences were identical. Phylogenetic tree of the ITS region sequences indicated that selected strains were classified into five clusters. The reciprocal homologies of the ITS region sequences ranged from 99 to 100%. The strains were also analyzed by RAPD with 20 arbitrary primers. Twelve primers were efficient to applying amplification of the genomic DNA. The sizes of the polymorphic fragments obtained were in the range of 200 to 2000 bp. RAPD and ITS analysis techniques were able to detect genetic variation among the tested strains. Experimental results suggested that IUM-1381, IUM-3914, IUM-1495 and AY-581431 strains were genetically very similar. Therefore, all IUM and NCBI gene bank strains of P. nebrodensis were genetically same with some variations.
基于九个不同商业栽培的白灵侧耳菌株的内部转录间隔区（ITS）和随机扩增多态性DNA（RAPD）对其分子系统发育进行了研究。在所选菌株的ITS区域序列中，发现其总长度在592至614碱基对之间。ITS1和ITS2区域的大小在不同菌株中分别为219至228碱基对和211至229碱基对。ITS2的序列比ITS1更具变异性，且5.8S序列区域相同。ITS区域序列的系统发育树表明所选菌株被分为五个簇。ITS区域序列的相互同源性在99%至100%之间。还用20个随机引物对这些菌株进行了RAPD分析。12个引物能有效扩增基因组DNA。获得的多态性片段大小在200至2000碱基对范围内。RAPD和ITS分析技术能够检测受试菌株之间的遗传变异。实验结果表明IUM-1381、IUM-3914、IUM-1495和AY-581431菌株在遗传上非常相似。因此，所有白灵侧耳的IUM和NCBI基因库菌株在遗传上基本相同，但存在一些变异。
