Dikovskaya Mariya A, Trunov Alexandr N, Chernykh Valeriy V, Korolenko Tatyana A
Institute of Physiology, Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk, Russia.
Int J Circumpolar Health. 2013 Aug 5;72. doi: 10.3402/ijch.v72i0.21087. eCollection 2013.
To investigate the possible role of cystatin C in eye biological fluids locally and in serum and lactoferrin revealing anti-tumor activity in eye tumor development.
The increased number of eye tumors was registered recently not only in the countries with high insolation, but also in the northern countries including Russia (11 cases per million of population). Search for new biological markers is important for diagnosis and prognosis in eye tumors. Cystatin C, an endogenous inhibitor of cysteine proteases, plays an important protective role in several tumors. Lactoferrin was shown to express anti-tumor and antiviral activities. It was hypothesized that cystatin C and lactoferrin could serve as possible biomarkers in the diagnosis of malignant and benign eye tumors.
A total of 54 patients with choroidal melanoma and benign eye tumors were examined (part of them undergoing surgical treatment). Serum, tear fluid and intraocular fluid samples obtained from the anterior chamber of eyes in patients with choroidal melanoma were studied.
Cystatin C concentration in serum and eye biological fluids was measured by commercial ELISA kits for human (BioVendor, Czechia); lactoferrin concentration--by Lactoferrin-strip D 4106 ELISA test systems (Vector-BEST, Novosibirsk Region, Russia).
Cystatin C concentration in serum of healthy persons was significantly higher as compared to tear and intraocular fluids. In patients with choroidal melanoma, increased cystatin C concentration was similar in tear fluid of both the eyes. Lactoferrin level in tear fluid of healthy persons was significantly higher than its serum level. Significantly increased lactoferrin concentration in tear fluid was noted in patients with benign and malignant eye tumors.
Increased level of cystatin C in tear fluid seems to be a possible diagnostic factor in the eye tumors studied. However, it does not allow us to differentiate between malignant and benign eye tumors. Similar changes were noted for lactoferrin in tear fluid.
研究胱抑素C在眼部生物体液局部以及血清中的可能作用，以及乳铁蛋白在眼部肿瘤发生发展过程中所呈现出的抗肿瘤活性。
近期不仅在日照强烈的国家，而且在包括俄罗斯在内的北方国家（每百万人口中有11例），眼部肿瘤的数量都有所增加。寻找新的生物标志物对于眼部肿瘤的诊断和预后至关重要。胱抑素C作为半胱氨酸蛋白酶的内源性抑制剂，在多种肿瘤中发挥着重要的保护作用。乳铁蛋白已被证明具有抗肿瘤和抗病毒活性。据推测，胱抑素C和乳铁蛋白可能作为恶性和良性眼部肿瘤诊断的潜在生物标志物。
共检查了54例脉络膜黑色素瘤和良性眼部肿瘤患者（部分患者接受了手术治疗）。对脉络膜黑色素瘤患者前房获取的血清、泪液和眼内液样本进行了研究。
采用商用人类ELISA试剂盒（捷克BioVendor公司）测定血清和眼部生物体液中胱抑素C的浓度；采用乳铁蛋白试纸条D 4106 ELISA检测系统（俄罗斯新西伯利亚地区Vector - BEST公司）测定乳铁蛋白的浓度。
健康人血清中胱抑素C的浓度显著高于泪液和眼内液。在脉络膜黑色素瘤患者中，双眼泪液中胱抑素C的浓度均升高。健康人泪液中乳铁蛋白水平显著高于其血清水平。在良性和恶性眼部肿瘤患者的泪液中，乳铁蛋白浓度显著升高。
泪液中胱抑素C水平升高似乎是所研究眼部肿瘤的一个可能诊断因素。然而，它无法使我们区分恶性和良性眼部肿瘤。泪液中乳铁蛋白也有类似变化。
