State Key Laboratory of Natural Medicines, PR China.
Carbohydr Polym. 2013 Oct 15;98(1):8-16. doi: 10.1016/j.carbpol.2013.04.057. Epub 2013 Apr 28.
Diabetes mellitus is a group of complicated metabolic disorders characterized by high blood glucose level and inappropriate insulin secreting capacity due to decreased glucose metabolism and pancreatic β cell mass or dysfunction of β cells. Thus, improving glucose metabolism and preserving β cell mass and function might be useful for the treatment of diabetes. In this study, a novel acidic polysaccharide LBP-s-1 extracted from Lycium barbarum L. was obtained by purification using macroporous resin and ion-exchanged column. Monosaccharide composition analysis indicated that LBP-s-1 was comprised of rhamnose, arabinose, xylose, mannose, glucose, galactose, galacturonic acid in the molar ratio of 1.00:8.34:1.25:1.26:1.91:7.05:15.28. The preliminary structure features of LBP-s-1 were investigated by FT-IR, (1)H NMR and (13)C NMR. In vitro and in vivo hypoglycemic experiments showed that LBP-s-1 had significant hypoglycemic effects and insulin-sensitizing activity through increasing glucose metabolism and insulin secretion and promoting pancreatic β cell proliferation. Preliminary mechanisms were also elucidated.
糖尿病是一组代谢紊乱的疾病，其特征为血糖水平升高和胰岛素分泌能力下降，这是由于葡萄糖代谢和胰岛β细胞数量或功能下降所致。因此，改善葡萄糖代谢和保护β细胞数量和功能可能对糖尿病的治疗有用。在这项研究中，我们通过大孔树脂和离子交换柱纯化从枸杞中获得了一种新型酸性多糖 LBP-s-1。单糖组成分析表明，LBP-s-1 由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖、半乳糖醛酸以摩尔比 1.00:8.34:1.25:1.26:1.91:7.05:15.28 组成。我们通过傅里叶变换红外光谱（FT-IR）、（1）H NMR 和（13）C NMR 对 LBP-s-1 的初步结构特征进行了研究。体外和体内降血糖实验表明，LBP-s-1 通过增加葡萄糖代谢和胰岛素分泌以及促进胰岛β细胞增殖，具有显著的降血糖和胰岛素增敏作用。我们还阐明了初步的作用机制。
