Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Int J Biol Macromol. 2012 Nov;51(4):417-22. doi: 10.1016/j.ijbiomac.2012.05.025. Epub 2012 May 28.
The polysaccharides from the fruits of Lycium barbarum have received considerable attention in previous publication, but the polysaccharides from the leaves were rarely reported. In the present work, four water-soluble polysaccharide fractions: LBP-I, LBP-II, LBP-III and LBP-IV isolated from L. barbarum leaves were purified through DEAE-Sephadex A-25. LBP-II and LBP-IV respectively showed one symmetrical peak on HPGPC with average molecular weight of 9.39 × 10⁴ Da and 4.18 × 10⁵ Da. UV and IR analysis of the two fractions showed the characteristics of acidic polysaccharides combined with polypeptides or proteins. GC analysis showed LBP-IV was mainly composed of rhamnose, arabinose, xylose, glucose and galactose with molar ratio of 1.61:3.82:3.44:7.54:1.00, and the uronic acid content was 47.68% (w/w) determined by sulfuric acid-carbazole method. ¹H and ¹³C NMR spectra of LBP-IV also showed the presence of carboxyl carbon and five anomeric carbons, and suggested there may be both α- and β-anomeric configurations in this fraction. Moreover, splenocyte proliferation activity assay showed that LBP-IV significantly enhanced the proliferation of splenocyte stimulated by ConA or LPS, indicating the fraction has the beneficial effect on immunostimulating activity.
从枸杞果实中提取的多糖在以前的出版物中受到了相当多的关注，但从枸杞叶中提取的多糖却很少有报道。在本研究中，从枸杞叶中分离得到的四种水溶性多糖组分：LBP-I、LBP-II、LBP-III 和 LBP-IV，通过 DEAE-Sephadex A-25 进行了纯化。LBP-II 和 LBP-IV 分别在 HPGPC 上呈现出一个对称峰，其平均分子量分别为 9.39×10⁴Da 和 4.18×10⁵Da。对这两个部分的 UV 和 IR 分析表明它们是酸性多糖与多肽或蛋白质结合的特征。GC 分析表明，LBP-IV 主要由鼠李糖、阿拉伯糖、木糖、葡萄糖和半乳糖组成，摩尔比为 1.61:3.82:3.44:7.54:1.00，糖醛酸含量为 47.68%（w/w），通过硫酸咔唑法测定。LBP-IV 的 ¹H 和 ¹³C NMR 图谱也表明存在羧基碳和五个端基碳，表明该部分可能同时存在α-和β-端基构型。此外，脾细胞增殖活性测定表明，LBP-IV 显著增强了 ConA 或 LPS 刺激的脾细胞增殖，表明该部分具有免疫刺激活性的有益作用。
