Biophysics Laboratory, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China; College of Pharmacy, Lanzhou University, Lanzhou, Gansu 730000, China.
Biophysics Laboratory, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China.
Int J Biol Macromol. 2021 Apr 15;176:352-363. doi: 10.1016/j.ijbiomac.2021.02.016. Epub 2021 Feb 4.
A water-soluble polysaccharide, designated as LBP-3, was isolated and purified from Lycium barbarum. Chemical analysis indicated that LBP-3 was composed of arabinose and galactose at a molar ratio of 1.00:1.56. The average molecular weight of LBP-3 was 6.74 × 104 Da. The structural features of LBP-3 were investigated by Fourier-transform infrared spectroscopy (FT-IR), methylation, and nuclear magnetic resonance (NMR). LBP-3 is a highly branched polysaccharide with a backbone of 1, 3-linked β-Galp, which is partially substituted at C-6. The branches contain 1, 5-linked α-Araf, 1, 6-linked β-Galp, 1, 3-linked α-Araf, and 1, 4-linked α-Araf. In vitro studies revealed that LBP-3 induced a concentration-dependent decrease in the levels of Aβ42/Aβ40 in N2a/APP695 cells. Proteomic analysis was conducted to investigate the potential molecular mechanism underlying the neuroprotective effect of LBP-3, and the results suggested that LBP-3 might have the potential for the treatment of AD.
从枸杞中分离纯化得到一种水溶性多糖，命名为 LBP-3。化学分析表明，LBP-3 由阿拉伯糖和半乳糖组成，摩尔比为 1.00:1.56。LBP-3 的平均分子量为 6.74×104 Da。通过傅里叶变换红外光谱（FT-IR）、甲基化和核磁共振（NMR）研究了 LBP-3 的结构特征。LBP-3 是一种高度支化的多糖，其主链为 1,3 连接的β-Galp，在 C-6 位部分取代。支链含有 1,5 连接的α-Araf、1,6 连接的β-Galp、1,3 连接的α-Araf 和 1,4 连接的α-Araf。体外研究表明，LBP-3 可诱导 N2a/APP695 细胞中 Aβ42/Aβ40 水平呈浓度依赖性降低。进行蛋白质组学分析以研究 LBP-3 神经保护作用的潜在分子机制，结果表明 LBP-3 可能具有治疗 AD 的潜力。
