Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIS T), 5 Hwarangno 14-gil, Seongbuk-gu, Seoul 136-791, Republic of Korea.
ACS Nano. 2013 Sep 24;7(9):8167-74. doi: 10.1021/nn403465a. Epub 2013 Aug 29.
The generation of nanosized probes often requires time-intensive and application-specific optimization processes that involve conjugating a nanoconstruct to a targeting moiety. Herein, we genetically modify apoferritin and generate a universal interface system composed of protein G and 6×His-tag. The resulting construct, conferred with modularity and high targeting efficiency, is applied toward two distinct applications in the detection of a pancreatic cancer biomarker and used to demonstrate its potential in the facile exchange of nanoprobe components.
纳米探针的生成通常需要耗费时间且需要针对特定应用进行优化，这涉及到将纳米结构与靶向部分进行缀合。在此，我们对脱铁铁蛋白进行基因修饰，并生成由蛋白 G 和 6×His 标签组成的通用接口系统。所得结构具有模块化和高靶向效率，应用于检测胰腺癌生物标志物的两种不同应用，并用于证明其在易于交换纳米探针组件方面的潜力。
