van Rijn H J, Linnemans W A, Boer P
J Bacteriol. 1975 Sep;123(3):1144-9. doi: 10.1128/jb.123.3.1144-1149.1975.
The localization of acid phosphatase (EC 3.1.3.2) in secreting protoplasts prepared from Saccharomyces cerevisiae is reported for the first time. Using a Gomori technique we were able to show acid phosphatase at those organelles in the protoplasts which are generally involved in the processes of biosynthesis and secretion of glycoproteins in eukaryotic cells.
首次报道了酿酒酵母分泌型原生质体中酸性磷酸酶(EC 3.1.3.2)的定位。通过戈莫里技术,我们能够在原生质体的那些细胞器中显示酸性磷酸酶,这些细胞器通常参与真核细胞中糖蛋白的生物合成和分泌过程。