Department of Dermatology, Kyushu University, Fukuoka, Japan; Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Dermatol Sci. 2013 Dec;72(3):311-9. doi: 10.1016/j.jdermsci.2013.07.005. Epub 2013 Aug 8.
S100 proteins belong to a family of calcium-binding proteins that regulate cell proliferation and differentiation. Despite our growing knowledge about the biology of S100 proteins in some human cancers, little is known about the expression of S100 family members in epidermal tumors and their clinical significance.
To determine the expression of S100A2, S100A4, S100A6, S100A7, as well as matrix metalloproteinases 9 (MMP9) in a spectrum of epidermal tumors with benign and malignant characteristics.
Immunohistological staining was performed for S100A2, S100A4, S100A6, S100A7, and MMP9 in 101 cases of various types of epidermal tumors, viz., squamous cell carcinoma (SCC), Bowen's disease (BD), actinic keratosis (AK), basal cell carcinoma (BCC), keratoacanthoma (KA), and seborrheic keratosis (SK). Thirteen specimens of normal skin (NS) served as control.
S100A2, S100A6, and S100A7 positive immunostaining was variably observed in different epidermal tumors. S100A4 staining was not observed in any epidermal tumors, but was clearly visible in dendritic cells. MMP9 immunostaining was positive only in 22/26 (84.62%) of SCC and 2/15 (13.33%) of BD cases. Expression of S100A2, S100A6, and S100A7 was increased in tumor cells compared to NS. However, only S100A6 expression was significantly associated with malignant transformation of epidermal tumors. Moreover, S100A6 expression was correlated with MMP9 expression in metastatic SCC.
Epidermal tumors show increased expression of S100A2 and S100A7 proteins. S100A4 may be a useful and distinct marker for epidermal dendritic cells. Expression of S100A6 and MMP9 in combination is associated with the development of SCC.
S100 蛋白属于钙结合蛋白家族，可调节细胞增殖和分化。尽管我们对某些人类癌症中 S100 蛋白的生物学特性有了更多的了解，但对于表皮肿瘤中 S100 家族成员的表达及其临床意义知之甚少。
确定 S100A2、S100A4、S100A6、S100A7 以及基质金属蛋白酶 9（MMP9）在具有良性和恶性特征的一系列表皮肿瘤中的表达情况。
采用免疫组织化学染色法检测 101 例各种类型表皮肿瘤（包括鳞状细胞癌[SCC]、鲍恩病[BD]、光化性角化病[AK]、基底细胞癌[BCC]、角化棘皮瘤[KA]和脂溢性角化病[SK]）和 13 例正常皮肤（NS）中 S100A2、S100A4、S100A6、S100A7 和 MMP9 的表达情况。
不同的表皮肿瘤中 S100A2、S100A6 和 S100A7 的阳性免疫染色呈不同程度的观察。S100A4 在任何表皮肿瘤中均未观察到染色，但在树突状细胞中清晰可见。MMP9 免疫染色仅在 26 例 SCC 中的 22 例（84.62%）和 15 例 BD 中的 2 例（13.33%）中为阳性。与 NS 相比，肿瘤细胞中 S100A2、S100A6 和 S100A7 的表达增加。然而，只有 S100A6 的表达与表皮肿瘤的恶性转化显著相关。此外，S100A6 的表达与转移性 SCC 中的 MMP9 表达相关。
表皮肿瘤中 S100A2 和 S100A7 蛋白表达增加。S100A4 可能是表皮树突状细胞的有用且独特的标志物。S100A6 和 MMP9 的表达与 SCC 的发生有关。
