Université de Bordeaux, Laboratoire de Biogenèse Membranaire (LBM), UMR 5200 CNRS-Université Bordeaux Segalen, 71 Avenue Edouard Bourlaux, 33883 Villenave-d'Ornon Cedex, France.
Phytochemistry. 2013 Dec;96:191-200. doi: 10.1016/j.phytochem.2013.08.002. Epub 2013 Aug 29.
Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots, GIPCs with three saccharides were apparently found to be major, whereas a series with two saccharides was dominant in Eudicots within a few notable exceptions. In plant cell cultures, GIPC polar heads appeared to bear a higher number of glycan units than in the tissue from which they originate. Perspectives are discussed in term of GIPC metabolism diversity and function of these lipids.
尽管糖基肌醇磷脂酰基神经酰胺（GIPC）是植物组织中的主要鞘脂类物质，但它们的结构特征仍知之甚少。这种信息的缺乏，特别是在极性头部方面，目前阻碍了对 GIPC 在生物系统中的功能的理解。这种情况促使我们对植物 GIPC 进行大规模分析：选择了 23 种来自不同进化群的植物物种来调查它们的总 GIPC 含量。提取 GIPC 并用负离子 MALDI 和 ESI 质谱法对其极性头部进行了表征。我们的数据揭示了在植物界中 GIPC 分布的惊人广泛的多样性，以及在绿藻和红藻中存在尚未报道的 GIPC 结构。在单子叶植物中，显然发现具有三个糖基的 GIPC 是主要的，而在几个显著的例外中，二糖系列在真双子叶植物中占主导地位。在植物细胞培养物中，GIPC 极性头部似乎比它们起源的组织具有更多的聚糖单位。从 GIPC 代谢多样性和这些脂质的功能方面进行了讨论。
