Song Cheng, Jiao Chunyan, Jin Qing, Chen Cunwu, Cai Yongping, Lin Yi
College of Biological and Pharmaceutical Engineering, West Anhui University, West Moon Island, Yunlu Bridge, Luan, 237012 Anhui People's Republic of China.
Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, Luan, People's Republic of China.
Physiol Mol Biol Plants. 2020 Jul;26(7):1425-1435. doi: 10.1007/s12298-020-00822-1. Epub 2020 May 9.
Nitrogen-containing compounds especially alkaloids are important medicinal ingredients in caulis dendrobii plants. Using solid-phase extraction coupled with liquid chromatography tandem mass spectrometry and multivariate data analysis methods, metabolic profiling of the nitrogen-containing compounds was established to distinguish and . Hundreds of nitrogen-containing compounds from the two caulis dendrobii were purified by the MCX cartridges. Some compounds were identified by high-resolution tandem mass spectrometry technology. Together with multivariate data analysis methods, comparative analysis of the metabolic profiling from two caulis dendrobii was conducted. A total of 133 nitrogen-containing compounds were identified, including amino acids, pyrrolidines, tropanes, pyrimidines, purines, indoles, piperidines, guanidines, quinolines, isoquinolines and terpenoids. Metabolic profiling analysis showed that the composition and contents of these chemical components were significantly different between and . Moreover, some components were species-specific, distributed in the two caulis dendrobii, such as pilosine, ternatusine, etc. Because alkaloids are mainly derived from amino acids via multistep biochemical reactions, the correlation analysis suggested that amino acids were partially associated with several types of components and significantly correlated with certain alkaloids. Arginine was extremely correlated with guanidines. Pyrimidines, purines and niacin-nicotinamide metabolic intermediates were associated with three independent networks. The results further enriched the chemical components currently identified from caulis dendrobii and provided a technical reference for detecting nitrogen-containing compounds in other medicinal plants.
含氮化合物尤其是生物碱是石斛属植物中的重要药用成分。采用固相萃取结合液相色谱串联质谱法及多元数据分析方法，建立了含氮化合物的代谢谱，以区分[具体区分对象未给出]。通过MCX柱从两种石斛中纯化出数百种含氮化合物。部分化合物通过高分辨率串联质谱技术进行了鉴定。结合多元数据分析方法，对两种石斛的代谢谱进行了比较分析。共鉴定出133种含氮化合物，包括氨基酸、吡咯烷、托烷、嘧啶、嘌呤、吲哚、哌啶、胍、喹啉、异喹啉和萜类化合物。代谢谱分析表明，这些化学成分的组成和含量在[具体比较对象未给出]之间存在显著差异。此外，一些成分具有种属特异性，分布于两种石斛中，如石斛碱、石斛宁等。由于生物碱主要通过多步生化反应由氨基酸衍生而来，相关性分析表明氨基酸与几种类型的成分部分相关，且与某些生物碱显著相关。精氨酸与胍类化合物高度相关。嘧啶、嘌呤和烟酸 - 烟酰胺代谢中间体与三个独立网络相关。该结果进一步丰富了目前从石斛中鉴定出的化学成分，并为检测其他药用植物中的含氮化合物提供了技术参考。
