T.A.L. Samy Centre for Plant Tissue Culture and Molecular Biology, Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai 600 034, Tamil Nadu, India.
Food Chem. 2013 Dec 15;141(4):4382-90. doi: 10.1016/j.foodchem.2013.06.108. Epub 2013 Jul 8.
Talinum triangulare (Jacq.) Willd is an erect, succulent, perennial herb belonging to the family Portulacaceae. Under extreme environmental conditions, the plant produces betalain pigments which get accumulated in the stem region. Hence, in the present study, the betaxanthin and betacyanin patterns from different samples of T. triangulare have been investigated by applying high-performance liquid chromatography photo-diode array detection (HPLC-PDA) coupled with positive ion electro-spray mass spectrometry. Two betacyanins and two betaxanthins were identified in aqueous methanolic extract of flower, stem and leaf. Betanin, isobetanin, immonium conjugates of betalamic acid with dopamine and tyrosine were elucidated. The total betalain content was estimated by photometric analysis. In vitro antioxidant activity for the betalain extract determined by various methods revealed potent scavenging ability. The current work may possibly be considered beneficial in utilisation of the plant T. triangulare as a natural colourant in food and beverage industries.
三指马兰（Jacq.）Willd 是马齿苋科马齿苋属的一种直立、肉质、多年生草本植物。在极端环境条件下，植物会产生甜菜红素类色素，并在茎部积累。因此，在本研究中，应用高效液相色谱光电二极管阵列检测（HPLC-PDA）结合正离子电喷雾质谱法，对来自不同三指马兰样品的甜菜黄素和甜菜红素模式进行了研究。在花、茎和叶的水甲醇提取物中鉴定出两种甜菜红素和两种甜菜黄素。阐明了甜菜碱、异甜菜碱、甜菜酰胺酸与多巴胺和酪氨酸的铵轭合物。通过分光光度分析估计总甜菜红素含量。通过各种方法测定的甜菜红素提取物的体外抗氧化活性显示出强大的清除能力。目前的工作可能有助于将植物三指马兰作为天然食用色素在食品和饮料行业中的应用。
