Animal Pathology Department, Veterinary Faculty, Santiago de Compostela University, 27002 Lugo, Spain.
Res Vet Sci. 2013 Dec;95(3):1031-5. doi: 10.1016/j.rvsc.2013.07.027. Epub 2013 Aug 3.
The aim of this work is to know the prevalence of Fasciola hepatica in 301 roe deer and in 676 beef cattle kept in an endemic area. Detection of antibodies was determined in roe deer using a homemade ELISA with excretory/secretory antigens (FhES) and a recombinant protein (FhrAPS). None of the deer passed eggs by faeces and none flukes in their livers were found. The seroprevalence of F. hepatica was 29% using FhES, with significantly higher values in the oldest ones (36%). Twenty-eight percent of the samples were positive to FhrAPS. Twenty-three percent of the cows eliminated eggs of F. hepatica and the seroprevalence was 67% using FhrAPS. No relationship between the seropositivity values of deer and cattle was demonstrated. The role of wild ruminants as reservoirs of F. hepatica is discussed. We encourage the use of ELISA to know the possibility of exposure to trematodes in wild ruminants.
本研究旨在了解在一个流行地区的 301 只獐和 676 头肉牛中肝片吸虫的流行情况。使用自制的含有排泄/分泌抗原（FhES）和重组蛋白（FhrAPS）的 ELISA 检测獐中的抗体。没有獐通过粪便排出虫卵，也没有在肝脏中发现片形吸虫。使用 FhES 检测，肝片吸虫的血清流行率为 29%，年龄最大的獐的阳性率显著更高（36%）。28%的样本对 FhrAPS 呈阳性。23%的牛排出肝片吸虫的虫卵，使用 FhrAPS 检测的血清流行率为 67%。獐和牛的血清阳性率之间没有显示出相关性。讨论了野生反刍动物作为肝片吸虫宿主的作用。我们鼓励使用 ELISA 来了解野生反刍动物感染吸虫的可能性。
