University of Bordeaux, ISVV, Unit of Enology, EA 4577, Villenave d'Ornon, France.
Int J Food Microbiol. 2013 Sep 2;166(2):331-40. doi: 10.1016/j.ijfoodmicro.2013.06.032. Epub 2013 Jul 7.
Temperate bacteriophages are a contributor of the genetic diversity in the lactic acid bacterium Oenococcus oeni. We used a classification scheme for oenococcal prophages based on integrase gene polymorphism, to analyze a collection of Oenococcus strains mostly isolated in the area of Bordeaux, which represented the major lineages identified through MLST schemes in the species. Genome sequences of oenococcal prophages were clustered into four integrase groups (A to D) which were related to the chromosomal integration site. The prevalence of each group was determined and we could show that members of the intB- and intC-prophage groups were rare in our panel of strains. Our study focused on the so far uncharacterized members of the intD-group. Various intD viruses could be easily isolated from wine samples, while intD lysogens could be induced to produce phages active against two permissive O. oeni isolates. These data support the role of this prophage group in the biology of O. oeni. Global alignment of three relevant intD-prophages revealed significant conservation and highlighted a number of unique ORFs that may contribute to phage and lysogen fitness.
温和噬菌体是乳球菌属 Oenococcus oeni 遗传多样性的贡献者。我们使用基于整合酶基因多态性的分类方案，对主要来自波尔多地区的一系列乳球菌菌株进行了分析，这些菌株代表了通过 MLST 方案在该物种中鉴定出的主要谱系。乳球菌噬菌体的基因组序列被聚类为四个整合酶组（A 至 D），它们与染色体整合位点有关。确定了每个组的流行率，我们可以表明，在我们的菌株组中，intB 和 intC 噬菌体组的成员很少。我们的研究集中在目前尚未表征的 intD 组成员上。各种 intD 病毒可以很容易地从葡萄酒样本中分离出来，而 intD 溶原菌可以被诱导产生针对两种允许的 O. oeni 分离物的噬菌体。这些数据支持了该噬菌体组在 O. oeni 生物学中的作用。三个相关的 intD 噬菌体的全局比对显示出显著的保守性，并突出了一些可能有助于噬菌体和溶原菌适应性的独特 ORF。
