Philippe Cécile, Jaomanjaka Fety, Claisse Olivier, Laforgue Rémi, Maupeu Julie, Petrel Melina, Le Marrec Claire
Univ. Bordeaux, ISVV, EA 4577, Unité de recherche Oenologie, F-33882 Villenave d'Ornon, France.
Univ. Bordeaux, ISVV, EA 4577, Unité de recherche Oenologie, F-33882 Villenave d'Ornon, France; Institut Polytechnique de Bordeaux, ISVV, EA 4577 Oenologie, F-33140 Villenave d'Ornon, France.
Int J Food Microbiol. 2017 Sep 18;257:138-147. doi: 10.1016/j.ijfoodmicro.2017.06.014. Epub 2017 Jun 17.
Oenophages have so far been mostly isolated from red wines under malolactic fermentation (MLF), and correspond to temperate or ex-temperate phages of Oenococcus oeni. Their genomes are clustered into 4 integrase gene sequence groups, which are also related to the chromosomal integration site. Our aims were to survey the occurrence of oenophages in a broader and more diverse collection of samples than those previously explored. Active phages were isolated from 33 out of 166 samples, which mostly originated from must and MLF. Seventy one phage lysates were produced and 30% were assigned to a novel group with unusual genomic characteristics, called unk. All unk members produced similar RAPD and DNA restriction patterns, were negative by PCR for the signature sequences previously identified in the integrase and endolysin genes of oenophages, and lacked any BamHI restriction site in their genome. The data support that development of additional and novel signature genes for assessing oenophage diversity is now required.
到目前为止，酒酒球菌噬菌体大多是从进行苹果酸-乳酸发酵（MLF）的红葡萄酒中分离出来的，它们属于酒酒球菌的温和噬菌体或前温和噬菌体。它们的基因组被聚类为4个整合酶基因序列组，这也与染色体整合位点有关。我们的目的是在比之前研究的样本更广泛、更多样化的样本集合中调查酒酒球菌噬菌体的存在情况。从166个样本中的33个分离出了活性噬菌体，这些样本大多来自葡萄汁和MLF。制备了71种噬菌体裂解物，其中30%被归为一个具有不寻常基因组特征的新组，称为unk组。所有unk组成员产生相似的随机扩增多态性DNA（RAPD）和DNA限制性图谱，通过聚合酶链反应（PCR）检测，它们在酒酒球菌噬菌体整合酶和内溶素基因中先前鉴定的特征序列呈阴性，并且其基因组中缺乏任何BamHI限制性位点。这些数据支持现在需要开发额外的新特征基因来评估酒酒球菌噬菌体的多样性。
