Biochemistry and Nanosciences Discipline, Defence Food Research Laboratory, Mysore, India.
Neurochem Res. 2013 Oct;38(10):2190-200. doi: 10.1007/s11064-013-1128-7. Epub 2013 Aug 31.
Oxidative stress mediates the cell damage in several ailments including neurodegenerative conditions. Ocimum sanctum is widely used in Indian ayurvedic medications to cure various ailments. The present study was carried out to investigate the antioxidant activity and neuroprotective effects of hydroalcoholic extract of O. sanctum (OSE) on hydrogen peroxide (H2O2)-induced oxidative challenge in SH-SY5Y human neuronal cells. The extract exhibited strong antioxidant activity against DPPH, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical and hydroxyl radicals with IC50 values of 395 ± 16.2, 241 ± 11.5 and 188.6 ± 12.2 μg/ml respectively, which could be due to high amount of polyphenols and flavonoids. The observed data demonstrates 41.5% cell survival with 100 μM H2O2 challenge for 24 h, which was restored to 73% by pre-treatment with OSE for 2 h. It also decreased the lactate dehydrogenase leakage and preserved the cellular morphology. Similarly OSE inhibited lipid peroxidation, DNA damage, reactive oxygen species generation and depolarization of mitochondrial membrane. The extract restored superoxide dismutase and catalase enzyme/protein levels and further downregulated HSP-70 over-expression. These findings suggest that OSE ameliorates H2O2 induced neuronal damage via its antioxidant defence mechanism and might be used to treat oxidative stress mediated neuronal disorders.
氧化应激介导几种疾病中的细胞损伤，包括神经退行性疾病。神圣罗勒（ Ocimum sanctum ）广泛用于印度阿育吠陀药物中，用于治疗各种疾病。本研究旨在探讨神圣罗勒（ Ocimum sanctum ）水醇提取物（ OSE ）对过氧化氢（ H2O2 ）诱导的 SH-SY5Y 人神经母细胞瘤氧化应激的抗氧化活性和神经保护作用。该提取物对 DPPH 、 2,2'- 联氮双（ 3-乙基苯并噻唑啉-6-磺酸）自由基和羟基自由基具有较强的抗氧化活性，IC50 值分别为 395 ± 16.2、241 ± 11.5 和 188.6 ± 12.2 μg/ml ，这可能是由于多酚和类黄酮含量高。观察到的数据表明，在 100 μM H2O2 作用 24 h 后，细胞存活率为 41.5% ，用 OSE 预处理 2 h 后可恢复至 73% 。它还降低了乳酸脱氢酶的渗漏并保持了细胞形态。同样，OSE 抑制脂质过氧化、DNA 损伤、活性氧生成和线粒体膜去极化。该提取物恢复了超氧化物歧化酶和过氧化氢酶的酶/蛋白水平，并进一步下调 HSP-70 的过度表达。这些发现表明，OSE 通过其抗氧化防御机制改善 H2O2 诱导的神经元损伤，可用于治疗氧化应激介导的神经元疾病。
