Pan Xuediao, Yang Zhouping, Yang Zhiying, Zhou Sigui, Zhang Haitao, Zang Linquan
Department of Pharmacology, New Drug Screening and Pharmacodynamics Evaluation Center, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Aug;33(8):1137-40.
To study the effect of apigenin on the proliferation and apoptosis of human lung cancer cell line NCI-H460.
NCI-H460 cells were cultured with different concentrations of apigenin, and MTT assay was used to evaluate the cell inhibition rates. Apoptosis of NCI-H460 cells was observed under a fluorescence microscope with Hoechst 33258 staining and quantified by flow cytometry using annexin V-FITC/PI stain. The expressions of apoptosis-related proteins Bax, Bcl-2 and caspase-3 were analyzed by Western blotting.
Apigenin causes concentration- and time-dependent inhibition of the proliferation of the cells. NCI-H460 cells treated with apigenin showed significant morphological changes of apoptosis, and the cell apoptotic rates increased as apigenin concentration increased. Western blotting demonstrated that apigenin increased the protein levels of Bax and caspase-3 and reduced the protein expression of Bcl-2.
Apigenin can inhibit the proliferation and induce apoptosis of NCI-H460 cells possibly by up-regulating expression of Bax and caspase-3 and down-regulating the expression of Bcl-2.
研究芹菜素对人肺癌细胞系NCI-H460增殖和凋亡的影响。
用不同浓度的芹菜素培养NCI-H460细胞，采用MTT法评估细胞抑制率。用Hoechst 33258染色在荧光显微镜下观察NCI-H460细胞的凋亡情况，并用annexin V-FITC/PI染色通过流式细胞术进行定量分析。通过蛋白质免疫印迹法分析凋亡相关蛋白Bax、Bcl-2和caspase-3的表达。
芹菜素对细胞增殖具有浓度和时间依赖性抑制作用。用芹菜素处理的NCI-H460细胞呈现出明显的凋亡形态变化，且细胞凋亡率随芹菜素浓度增加而升高。蛋白质免疫印迹法表明，芹菜素增加了Bax和caspase-3的蛋白水平，降低了Bcl-2的蛋白表达。
芹菜素可能通过上调Bax和caspase-3的表达以及下调Bcl-2的表达来抑制NCI-H460细胞的增殖并诱导其凋亡。
