番木瓜叶提取物能增加血小板计数吗？一项在小鼠模型中的实验研究。
Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model.
作者信息
Dharmarathna Sinhalagoda Lekamlage Chandi Asoka, Wickramasinghe Susiji, Waduge Roshitha Nilmini, Rajapakse Rajapakse Peramune Veddikkarage Jayanthe, Kularatne Senanayake Abeysinghe Mudiyanselage
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka.
出版信息
Asian Pac J Trop Biomed. 2013 Sep;3(9):720-4. doi: 10.1016/S2221-1691(13)60145-8.
OBJECTIVE
To investigate the potential role of fresh Carica papaya (C. papaya) leaf extract on haematological and biochemical parameters and toxicological changes in a murine model.
METHODS
In total 36 mice were used for the trial. Fresh C. papaya leaf extract [0.2 mL (2 g)/mouse] was given only to the test group (18 mice). General behavior, clinical signs and feeding patterns were recorded. Blood and tissue samples were collected at intervals. Haematological parameters including platelet, red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), serum biochemistry including serum creatinine, serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) were determined. Organs for possible histopathological changes were examined.
RESULTS
Neither group exhibited alteration of behavior or reduction in food and water intake. Similarly, no significant changes in SGOT, SGPT and serum creatinine levels were detected in the test group. Histopathological organ changes were not observed in either group of mice except in three liver samples of the test group which had a mild focal necrosis. The platelet count (11.33±0.35)×10⁵/µL (P=0.00004) and the RBC count (7.97±0.61)×10⁶/µL (P=0.00003) were significantly increased in the test group compared to that of the controls. However, WBC count and PCV (%) values were not changed significantly in the test group. The platelet count in the test group started to increase significantly from Day 3 (3.4±0.18×10⁵/µL), reaching almost a fourfold higher at Day 21 (11.3×10⁵/µL), while it was 3.8×10⁵/µL and 5.5×10⁵/µL at Day 3 and Day 21 respectively in the control. Likewise, the RBC count in the test group increased from 6×10⁶/µL to 9×10⁶/ µL at Day 21 while it remained near constant in the control group (6×10⁶/µL).
CONCLUSIONS
Fresh C. papaya leaf extract significantly increased the platelet and RBC counts in the test group as compared to controls. Therefore, it is very important to identify those chemicals of C. papaya leaves as it can be recommended to be used as a medication to boost thrombopoiesis and erythropoiesis in humans and in animals in which these cell lineages have been compromised.
目的
研究新鲜番木瓜叶提取物对小鼠血液学和生化指标以及毒理学变化的潜在作用。
方法
总共36只小鼠用于该试验。仅给试验组（18只小鼠）给予新鲜番木瓜叶提取物[0.2 mL(2 g)/只小鼠]。记录一般行为、临床症状和进食模式。定期采集血液和组织样本。测定血液学指标，包括血小板、红细胞（RBC）、白细胞（WBC）、血细胞比容（PCV），血清生化指标，包括血清肌酐、血清谷草转氨酶（SGOT）和血清谷丙转氨酶（SGPT）。检查可能发生组织病理学变化的器官。
结果
两组均未出现行为改变或食物和水摄入量减少。同样，试验组中未检测到SGOT、SGPT和血清肌酐水平有显著变化。除试验组的三个肝脏样本有轻度局灶性坏死外，两组小鼠均未观察到组织病理学器官变化。与对照组相比，试验组的血小板计数(11.33±0.35)×10⁵/µL（P = 0.00004）和红细胞计数(7.97±0.61)×10⁶/µL（P = 0.00003）显著增加。然而，试验组的白细胞计数和PCV(%)值没有显著变化。试验组的血小板计数从第3天(3.4±0.18×10⁵/µL)开始显著增加，在第21天达到几乎四倍高(11.3×10⁵/µL)，而对照组在第3天和第21天分别为3.8×10⁵/µL和5.5×10⁵/µL。同样，试验组的红细胞计数在第21天从6×10⁶/µL增加到9×10⁶/µL，而对照组保持在接近恒定水平(6×10⁶/µL)。
结论
与对照组相比，新鲜番木瓜叶提取物显著增加了试验组的血小板和红细胞计数。因此，鉴定番木瓜叶中的那些化学物质非常重要，因为它可以被推荐用作药物，以促进人类和这些细胞谱系受损的动物的血小板生成和红细胞生成。
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