Khajuria Atul, Praharaj Ashok Kumar, Kumar Mahadevan, Grover Naveen
PhD Scholar, Department of Microbiology, Armed Forces Medical College , Pune -411040, India .
J Clin Diagn Res. 2013 Jul;7(7):1328-31. doi: 10.7860/JCDR/2013/5509.3137. Epub 2013 Jul 1.
The present study was undertaken to detect the prevalence of the blaNDM-1 metallo beta lactamases (MBLs) in the isolates of Pseudomonas aeruginosa, which were recovered from various clinical samples from hospitalized patients in a tertiary care centre in Pune, India.
A total of 200 isolates of P. aeruginosa which were obtained from various clinical samples were subjected to antibiotic susceptibility testing by the disc-diffusion method and their MICs were determined by the Vitek - 2 Automated Antimicrobial Identification and Susceptibility Testing System against imipenem, meropenem, ticarcillin, amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, moxifloxacin, tigecycline, trimethoprim/sulfamethoxazole, ampicillin/sulbactam, piperacillin/tazobactam, cefoperazone/sulbactam, cefepime, tetracycline, ceftazidime, ceftriaxone and colistin. Their MICs were also determined by the Etest method against imipenem, meropenem, piperacillin, tobramycin, ceftazidime, tigecycline and colistin. The presence of blaNDM-1 was detected by PCR and it was confirmed by sequencing the gene which was present in the isolates which exhibited carbapenem resistance. The experimental transferability of the plasmids which carried blaNDM-1 was determined by using E. coli J53 as the recipient.
In the present study, four isolates of P. aeruginosa, which carried the blaNDM-1 gene, were resistant to imipenem and meropenem. These blaNDM-1 carrying isolates remained susceptible to colistin. The plasmid carrying blaNDM-1 was successfully transferred from the four isolates to E. coli J53 recipients.
We are reporting the emergence of the P. aeruginosa carrying NDM-1gene, which exhibited resistance to imipenem and meropenem, for the first time from India.
本研究旨在检测从印度浦那一家三级护理中心住院患者的各种临床样本中分离出的铜绿假单胞菌菌株中blaNDM - 1金属β - 内酰胺酶（MBLs）的流行情况。
从各种临床样本中获得的总共200株铜绿假单胞菌菌株，采用纸片扩散法进行抗生素敏感性测试，并使用Vitek - 2自动抗菌鉴定和药敏测试系统测定它们对亚胺培南、美罗培南、替卡西林、阿米卡星、庆大霉素、妥布霉素、环丙沙星、左氧氟沙星、莫西沙星、替加环素、甲氧苄啶/磺胺甲恶唑、氨苄西林/舒巴坦、哌拉西林/他唑巴坦、头孢哌酮/舒巴坦、头孢吡肟、四环素、头孢他啶、头孢曲松和黏菌素的最低抑菌浓度（MIC）。还通过Etest法测定它们对亚胺培南、美罗培南、哌拉西林、妥布霉素、头孢他啶、替加环素和黏菌素的MIC。通过PCR检测blaNDM - 1的存在，并通过对表现出碳青霉烯耐药性的分离株中存在的基因进行测序来确认。使用大肠杆菌J53作为受体来测定携带blaNDM - 1的质粒的实验转移性。
在本研究中，携带blaNDM - 1基因的4株铜绿假单胞菌菌株对亚胺培南和美罗培南耐药。这些携带blaNDM - 1的分离株对黏菌素仍敏感。携带blaNDM - 1的质粒成功地从这4株分离株转移到了大肠杆菌J53受体中。
我们首次从印度报道了携带NDM - 1基因且对亚胺培南和美罗培南耐药的铜绿假单胞菌的出现。
