Di Caprio Giuseppe, Schaak Diane, Schonbrun Ethan
Rowland Institute at Harvard, Harvard University, 100 E. Land Boulevard, Cambridge MA, USA.
Biomed Opt Express. 2013 Jul 31;4(8):1486-93. doi: 10.1364/BOE.4.001486. eCollection 2013.
We present an imaging system that collects hyperspectral images of cells travelling through a microfluidic channel. Using a single monochrome camera and a linear variable bandpass filter (LVF), the system captures a bright field image and a set of hyperspectral fluorescence images for each cell. While the bandwidth of the LVF is 20 nm, we have demonstrated that we can determine the peak wavelength of a fluorescent object's emission spectrum with an accuracy of below 3 nm. In addition, we have used this system to capture fluorescence spectra of individual spatially resolved cellular organelles and to spectrally resolve multiple fluorophores in individual cells.
我们展示了一种成像系统，该系统可收集通过微流体通道的细胞的高光谱图像。利用单个单色相机和线性可变带通滤波器（LVF），该系统为每个细胞捕获一幅明场图像和一组高光谱荧光图像。虽然LVF的带宽为20纳米，但我们已证明能够以低于3纳米的精度确定荧光物体发射光谱的峰值波长。此外，我们已使用该系统来捕获单个空间分辨细胞器的荧光光谱，并在单个细胞中对多个荧光团进行光谱分辨。
