[大孔吸附树脂法纯化百蕊草提取物中的总黄酮]
[Purification of total flavonoids from the extracts of Thesium Chinese with macroporous adsorption resin].
作者信息
Chu Xiao-Qin, Hu Ye-Qing, Yue Lei
机构信息
Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
出版信息
Zhong Yao Cai. 2013 Mar;36(3):478-81.
OBJECTIVE
To optimize the process parameters for purifying total flavonoids from Thesium chinese with D101 macroporous adsorption resin.
METHODS
Purification technology of total flavonoids from the extracts of Thesium chinese was investigated by dynamic adsorption method with the transfer rates and the purity of total flavonoids as indexes.
RESULTS
The optimal purifying conditions were as follows: sample concentration was 2.259 mg/mL with a speed of 1 BV/h, eluent concentration was 70% alcohol with a speed of 1BV/h. After purification, the transfer rate of total flavonoids was 94.44% and the purity of total flavonoids achieved 12.45%, which was 4 times of that by coarse extraction (2.91%).
CONCLUSION
The purification technology is simple, stable and can significantly improve the contents of total flavoids in extracts.
目的
优化用D101大孔吸附树脂纯化百蕊草总黄酮的工艺参数。
方法
以总黄酮转移率和纯度为指标，采用动态吸附法研究百蕊草提取物中总黄酮的纯化工艺。
结果
最佳纯化条件如下：样品浓度为2.259mg/mL，流速为1BV/h，洗脱剂浓度为70%乙醇，流速为1BV/h。纯化后，总黄酮转移率为94.44%，总黄酮纯度达到12.45%，是粗提物（2.91%）的4倍。
结论
该纯化工艺简单、稳定，能显著提高提取物中总黄酮的含量。
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