Li Yan-Rong, Yan Li-Xin, Feng Wei-Hong, Li Chun, Wang Zhi-Min
Zhong Yao Cai. 2014 Apr;37(4):670-4.
To optimize the extraction and purification technologies of total flavonoids from Aconitum tanguticum whole plant.
With the content of total flavonoids as index, the optimum extraction conditions for the concentration, volume of alcohol, extracting time and times were selected by orthogonal optimized; Comparing the adsorption quantity (mg/g) and resolution (%), four kinds of macroporous adsorption resins including D101, AB-8, X-5 and XAD-16 were investigated for the enrichment ability of total flavonoids from Aconitum tanguticum; Concentration and pH value of sample, sampling amount, elution solvent and loading and elution velocity for the optimum adsorption resin were determined.
The content of total flavonoids in Aconitum tanguticum was about 4.39%; The optimum extraction technique was 70% alcohol reflux extraction for three times,each time for one hour, the ratio of material and liquid was 1:10 (w/v); The optimum purification technology was: using XAD-16 macroporous resin, the initial concentration of total flavonoids of Aconitum tanguticum was 8 mg/mL, the sampling amount was 112 mg/g dry resin, the pH value was 5, the loading velocity was 3 mL/min, the elution solvent was 70% ethanol and the elution velocity was 5 mL/min. Under the optimum conditions, the average content of total flavonoids was raised from 4.39% to 46.19%.
The optimum extraction and purification technologies for total flavonoids of Aconitum tanguticum were suitable for industrial production for its simplicity and responsibility.
优化唐古特乌头全草总黄酮的提取与纯化工艺。
以总黄酮含量为指标，通过正交试验优选乙醇浓度、体积、提取时间及次数等最佳提取条件；比较D101、AB - 8、X - 5和XAD - 16四种大孔吸附树脂对唐古特乌头总黄酮的富集能力，考察其吸附量（mg/g）及解析率（%）；确定最佳吸附树脂的样品浓度、pH值、上样量、洗脱剂以及上样和洗脱流速。
唐古特乌头中总黄酮含量约为4.39%；最佳提取工艺为70%乙醇回流提取3次，每次1小时，料液比1:10（w/v）；最佳纯化工艺为：采用XAD - 16大孔树脂，唐古特乌头总黄酮初始浓度8 mg/mL，上样量112 mg/g干树脂，pH值5，上样流速3 mL/min，洗脱剂为70%乙醇，洗脱流速5 mL/min。在最佳条件下，总黄酮平均含量由4.39%提高至46.19%。
唐古特乌头总黄酮最佳提取与纯化工艺简便可行，适合工业化生产。
