A G Deepa, Janardanan-Nair Bindu, B R Varun
Assistant Professor, Department of Oral and Maxillofacial Pathology , Sree Mookambika Institute of Dental Sciences, Kulasekharam, Tamil Nadu, India.
Consultant oral pathologist, Dr. Vivek's Dental Clinic, Vazhuthacaud, Trivandrum, Kerala, India.
J Clin Exp Dent. 2017 Dec 1;9(12):e1418-e1424. doi: 10.4317/jced.54213. eCollection 2017 Dec.
Podoplanin is a type I transmembrane sialomucin-like glycoprotein that is specifically expressed in lymphatic endothelial cells. Studies have shown that assessment of podoplanin expression in the epithelial cells can be used to predict the malignant transformation of potentially malignant disorders and the metastatic tendency of primary head and neck squamous cell carcinoma. The aim of our study was to compare the expression of podoplanin in oral leukoplakia, oral submucous fibrosis and oral squamous cell carcinoma with that in normal buccal mucosa by immunohistochemical methods.
Immunohistochemical expression of podoplanin was analyzed in 20 cases each of oral leukoplakia, oral submucous fibrosis, oral squamous cell carcinoma and normal buccal mucosa, with monoclonal antibody D2-40. The expression of podoplanin was graded from grade 0-4.
There was a statistically significant upregulation of the grades of podoplanin expression in oral squamous cell carcinoma(100%), oral submucous fibrosis (90%) and oral leukoplakia (65%) when compared to that in normal mucosa(35%). Podoplanin expression increased with decrease in grades of differentiation in oral squamous cell carcinoma . Podoplanin expression in the samples of oral submucous fibrosis was higher than that in oral leukoplakia.
Evaluation of podoplanin expression in the epithelial cells of oral dysplastic lesions may provide valuable information to predict their risk of malignant transformation. Immunohistochemistry, Oral leukoplakia, Oral submucous fibrosis, Podoplanin, Squamous cell carcinoma.
血小板内皮细胞黏附分子是一种I型跨膜唾液酸黏蛋白样糖蛋白，在淋巴管内皮细胞中特异性表达。研究表明，评估上皮细胞中血小板内皮细胞黏附分子的表达可用于预测潜在恶性疾病的恶变以及原发性头颈部鳞状细胞癌的转移倾向。我们研究的目的是通过免疫组织化学方法比较血小板内皮细胞黏附分子在口腔白斑、口腔黏膜下纤维化和口腔鳞状细胞癌中的表达与正常颊黏膜中的表达。
采用单克隆抗体D2-40，对20例口腔白斑、口腔黏膜下纤维化、口腔鳞状细胞癌及正常颊黏膜组织进行血小板内皮细胞黏附分子免疫组织化学表达分析。血小板内皮细胞黏附分子的表达按0-4级分级。
与正常黏膜（35%）相比，口腔鳞状细胞癌（100%）、口腔黏膜下纤维化（90%）和口腔白斑（65%）中血小板内皮细胞黏附分子表达分级有统计学意义的上调。在口腔鳞状细胞癌中，血小板内皮细胞黏附分子表达随分化程度降低而增加。口腔黏膜下纤维化样本中血小板内皮细胞黏附分子的表达高于口腔白斑。
评估口腔发育异常病变上皮细胞中血小板内皮细胞黏附分子的表达可能为预测其恶变风险提供有价值的信息。免疫组织化学、口腔白斑、口腔黏膜下纤维化、血小板内皮细胞黏附分子、鳞状细胞癌。
