Wright State University Boonshoft Schoolof Medicine, 3640 Col. Glenn Highway, Dayton, Ohio45435, USA.
Bone Joint Res. 2013 Sep 10;2(9):186-92. doi: 10.1302/2046-3758.29.2000196. Print 2013.
The goals of this study were: 1) to determine if high-fat diet (HFD) feeding in female mice would negatively impact biomechanical and histologic consequences on the Achilles tendon and quadriceps muscle; and 2) to investigate whether exercise and branched-chain amino acid (BCAA) supplementation would affect these parameters or attenuate any negative consequences resulting from HFD consumption.
We examined the effects of 16 weeks of 60% HFD feeding, voluntary exercise (free choice wheel running) and BCAA administration in female C57BL/6 mice. The Achilles tendons and quadriceps muscles were removed at the end of the experiment and assessed histologically and biomechanically.
HFD feeding significantly decreased the Achilles tendon modulus without histological alterations. BCAA administration significantly decreased the stiffness of Achilles tendons in the exercised normal diet mice. Exercise partially ameliorated both the weight gain and glucose levels in the HFD-fed mice, led to a significant decrease in the maximum load of the Achilles tendon, and an increase in the average fibril diameter of the quadriceps femoris muscle. There were significant correlations between body weight and several biomechanical properties, demonstrating the importance of controlling obesity for maintaining healthy tendon properties.
In summary, this study showed a significant impact of obesity and body weight on tendon biomechanical properties with limited effects of exercise and BCAAs. Cite this article: Bone Joint Res 2013;2:186-92.
本研究的目的为：1）确定高脂肪饮食（HFD）喂养对雌性小鼠跟腱和四头肌的生物力学和组织学后果是否有负面影响；2）研究运动和支链氨基酸（BCAA）补充是否会影响这些参数或减轻 HFD 摄入产生的任何负面影响。
我们检测了 16 周 60%HFD 喂养、自由选择轮跑运动和 BCAA 给药对雌性 C57BL/6 小鼠的影响。实验结束时取出跟腱和四头肌，进行组织学和生物力学评估。
HFD 喂养显著降低了跟腱的模量，而没有组织学改变。BCAA 给药显著降低了运动正常饮食小鼠跟腱的刚度。运动部分改善了 HFD 喂养小鼠的体重增加和血糖水平，显著降低了跟腱的最大负荷，并增加了股四头肌的平均纤维直径。体重与几种生物力学特性之间存在显著相关性，表明控制肥胖对于维持健康的肌腱特性非常重要。
综上所述，本研究表明肥胖和体重对肌腱生物力学特性有显著影响，运动和 BCAA 的影响有限。
