Bergesen O, Schjønsby H, Schjerven L
Medical Dept., Aker University Hospital, Oslo, Norway.
Scand J Gastroenterol. 1990 Aug;25(8):819-24. doi: 10.3109/00365529008999221.
To investigate whether bile reduces the amount of vitamin B12 bound by intrinsic factor (IF) in the intestinal juice in vivo, choledochocolic fistulae were made in 9 rats, and 10 rats were sham-operated. Small-intestinal juice was collected 1 h after gastric instillation of 57CoB12. The percentage of 57CoB12 bound by IF in the intestinal juice was markedly increased in fistula rats (median, 86%; range, 75-91%) as compared with sham-operated rats (35%; 12-50%) (p less than 0.001). In a second experiment we investigated whether bile enhances the intestinal uptake of the IF-B12 complex. Aliquots of the radioactive intestinal juice collected from the sham- and fistula-operated rats were separately instilled into tied intestinal loops created in 19 rats. The percentage of the instilled radioactivity taken up per 10 cm of the loop was significantly higher when juice from fistula-operated rats was instilled (median, 12.4; range, 4.3-18.7) than when juice from sham-operated rats was instilled (3.3; 1.4-6.2) (p less than 0.001).
为研究胆汁是否会降低体内肠液中内因子（IF）结合的维生素B12量，对9只大鼠制作了胆总管结肠瘘，另10只大鼠做假手术。在向胃内注入57CoB12 1小时后收集小肠液。与假手术大鼠（35%；12 - 50%）相比，瘘管大鼠肠液中IF结合的57CoB12百分比显著增加（中位数为86%；范围为75 - 91%）（p < 0.001）。在第二个实验中，我们研究了胆汁是否会增强肠道对IF - B12复合物的摄取。将从假手术和瘘管手术大鼠收集的放射性肠液等分试样分别注入19只大鼠制作的结扎肠袢中。注入瘘管手术大鼠肠液时，每10厘米肠袢摄取的注入放射性百分比显著高于注入假手术大鼠肠液时（中位数为12.4；范围为4.3 - 18.7）（3.3；1.4 - 6.2）（p < 0.001）。
