Bergesen O, Schjønsby H, Schjerven L
Medical Dept. B, Aker University Hospital, Oslo, Norway.
Scand J Gastroenterol. 1988 May;23(4):471-6. doi: 10.3109/00365528809093896.
To investigate whether vitamin B12 malabsorption in rats with choledochocolic fistulae is caused by bacterial overgrowth, we studied intestinal bacterial metabolic activity in choledochocolic fistula-operated rats, self-filling blind-loop-operated rats, and sham-operated rats. Fistula-operated rats had a moderate indicanuria compared with sham-operated ones, whereas the faecal excretion of nitrogen was unchanged. There was no difference in the amounts of radioactivity recovered in sediments of intestinal contents after an oral dose of 57CoB12, indicating unaltered bacterial uptake of vitamin B12 in fistula rats. The 14C-xylose breath test showed bacterial overgrowth in blind-loop rats only. Neomycin treatment of fistula rats diminished the excretion of indican but did not alter the absorption of vitamin B12. The results suggest moderately increased activity of indole-producing bacteria in fistula rats, but they do not support the hypothesis that vitamin B12 malabsorption is caused by bacterial overgrowth.
为了研究胆总管胆瘘大鼠维生素B12吸收不良是否由细菌过度生长引起，我们对胆总管胆瘘手术大鼠、自行充盈盲袢手术大鼠和假手术大鼠的肠道细菌代谢活性进行了研究。与假手术大鼠相比，胆瘘手术大鼠有中度的尿蓝母尿症，而粪便氮排泄量未改变。口服57CoB12后，肠道内容物沉淀物中回收的放射性量没有差异，表明胆瘘大鼠中细菌对维生素B12的摄取未改变。14C-木糖呼气试验仅显示盲袢大鼠有细菌过度生长。用新霉素治疗胆瘘大鼠可减少尿蓝母的排泄，但不改变维生素B12的吸收。结果表明胆瘘大鼠中产生吲哚的细菌活性适度增加，但不支持维生素B12吸收不良是由细菌过度生长引起的这一假说。
