Sharma R, Woldehiwet Z
University of Liverpool, Department of Veterinary Pathology, Leahurst, Neston, Wirral, Great Britain.
Vet Microbiol. 1990 Jun;23(1-4):267-72. doi: 10.1016/0378-1135(90)90157-q.
Twenty-four 6-8-week-old conventionally reared lambs were inoculated intranasally and intratracheally with bovine respiratory syncytial virus. Infected lambs showed mild clinical signs characterized by slight serous nasal discharge, coughing, lachrymation and bronchovascular sounds on the middle part of the lung 5-9 days post-inoculation (PI). Virus was isolated in nasal swabs from 9 of 24 lambs between 3 and 7 days PI. However, virus was recovered from tracheal and lung tissue of all lambs killed between 3 and 11 days PI. Virus-specific antibodies appeared as early 6 days PI but high titres were attained 14-21 days PI. Lungs of lambs killed on different days PI had multifocal areas of consolidation. There was an increase of lymphocytes with a T-suppressor cell marker and a decrease in those with a T-helper marker in lung lavages obtained 5 days PI.
将24只6 - 8周龄常规饲养的羔羊经鼻内和气管内接种牛呼吸道合胞病毒。接种后5 - 9天，受感染的羔羊表现出轻微的临床症状，特征为轻微浆液性鼻漏、咳嗽、流泪以及肺部中部的支气管血管音。在接种后3至7天，从24只羔羊中的9只的鼻拭子中分离出病毒。然而，在接种后3至11天处死的所有羔羊的气管和肺组织中都检测到了病毒。病毒特异性抗体在接种后6天就出现，但在接种后14 - 21天达到高滴度。在不同接种后天数处死的羔羊的肺有多处实变区域。在接种后5天获得的肺灌洗液中，具有T抑制细胞标志物的淋巴细胞增加，而具有T辅助标志物的淋巴细胞减少。
