Sharma R, Woldehiwet Z
University of Liverpool, Department of Veterinary Pathology, Neston, Wirral, U.K.
J Comp Pathol. 1990 Nov;103(4):411-20. doi: 10.1016/s0021-9975(08)80029-1.
Groups of conventionally reared, 6- to 8-week-old lambs were inoculated intranasally and intratracheally with either bovine respiratory syncytial virus (BRSV) or BRSV followed by Pasteurella haemolytica 6 days later or with P. haemolytica alone. Lambs infected with P. haemolytica 6 days after experimental infection with BRSV had significantly higher disease scores, greater magnitudes of fever, and higher mortality rates than those infected with BRSV alone or with P. haemolytica alone (P less than 0.05). Mononuclear cells in the peripheral blood and lung lavage obtained from the BRSV-infected lambs were more susceptible to P. haemolytica cytotoxin than those obtained from control lambs (P less than 0.05). P. haemolytica was isolated in significantly greater numbers from lambs infected with BRSV and P. haemolytica than from those infected with P. haemolytica alone (P less than 0.05).
将6至8周龄的常规饲养羔羊分成几组，通过鼻内和气管内接种牛呼吸道合胞病毒（BRSV），或先接种BRSV，6天后再接种溶血巴斯德氏菌，或仅接种溶血巴斯德氏菌。实验感染BRSV 6天后感染溶血巴斯德氏菌的羔羊，其疾病评分显著更高，发热程度更高，死亡率也高于仅感染BRSV或仅感染溶血巴斯德氏菌的羔羊（P<0.05）。与对照组羔羊相比，从感染BRSV的羔羊外周血和肺灌洗中获得的单核细胞对溶血巴斯德氏菌细胞毒素更敏感（P<0.05）。与仅感染溶血巴斯德氏菌的羔羊相比，感染BRSV和溶血巴斯德氏菌的羔羊分离出的溶血巴斯德氏菌数量显著更多（P<0.05）。
