Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la Biomasse, Ecole Normale Supérieure de Kouba , BP 92, 16050 Kouba, Alger , Algérie.
Braz J Microbiol. 2011 Jul;42(3):835-45. doi: 10.1590/S1517-83822011000300002. Epub 2011 Sep 1.
A moderately halophilic actinomycete strain designated AH97 was isolated from a saline Saharan soil, and selected for its antimicrobial activities against bacteria and fungi. The AH97 strain was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Actinoalloteichus. Analysis of the 16S rDNA sequence of strain AH97 showed a similarity level ranging between 95.8% and 98.4% within Actinoalloteichus species, with A. hymeniacidonis the most closely related. The comparison of the physiological characteristics of AH97 with those of known species of Actinoalloteichus showed significant differences. Strain AH97 showed an antibacterial and antifungal activity against broad spectrum of microorganisms known to be human and plant pathogens. The bioactive compounds were extracted from the filtrate culture with n-butanol and purified using thin layer chromatography and high pressure liquid chromatography procedures. Two active products were isolated, one hydrophilic fraction (F1) and another hydrophobic (F2). Ultraviolet-visible, infrared, mass and (1)H and (13)C nuclear magnetic resonance spectroscopy studies suggested that these molecules were the dioctyl phthalate (F2) and an aminoglycosidic compound (F1).
一株中度嗜盐放线菌 AH97 从撒哈拉沙漠的盐土中分离得到，因其对细菌和真菌的抗菌活性而被选中。AH97 菌株通过形态学、化学分类学和系统发育分析被鉴定为 Actinoalloteichus 属。菌株 AH97 的 16S rDNA 序列分析表明，在 Actinoalloteichus 种内，其相似度在 95.8%至 98.4%之间，与 A. hymeniacidonis 最为接近。与已知的 Actinoalloteichus 种的生理特征比较表明，AH97 株存在显著差异。AH97 株对广谱的已知人类和植物病原菌具有抗菌和抗真菌活性。生物活性化合物用正丁醇从滤液培养物中提取，并通过薄层层析和高压液相色谱程序进行纯化。分离出两种活性产物，一种亲水部分（F1）和另一种疏水部分（F2）。紫外可见、红外、质谱和（1）H 和（13）C 核磁共振波谱研究表明，这些分子是邻苯二甲酸二辛酯（F2）和氨基糖苷类化合物（F1）。
