Zhang Xiaoying, Song Chunfeng, Bai Yan, Hu Jiangchun, Pan Huaqi
Institute of Applied Ecology, Chinese Academy of Sciences, South Building Rm 308, 72 Wenhua Road, Shenhe District, Shenyang, 110016 China.
3 Biotech. 2021 Jun;11(6):283. doi: 10.1007/s13205-021-02846-0. Epub 2021 May 21.
A new deep-sea-derived actinomycete 12A22 was isolated from the sediment of the South China Sea which showed potential cytotoxic and antimicrobial activities. The actinomycete was identified as by investigating morphological characteristics and phylogenetic analyses based on its 16S rRNA gene sequence. Two compounds, cyclo-(-Pro--Pro--Tyr--Tyr) () and 2-hydroxyethyl-3-methyl-1,4-naphthoquinone (), were isolated and characterized from the fermentation broth of the strain 12A22. Compound exhibited significant inhibitory activities against a variety of phytopathogenic fungi ( f. sp. , , and ) and Gram-positive bacterium (). In particular, this compound showed better antifungal activity against than positive control amphotericin B. Besides, compound showed moderate cytotoxic activity against human breast cancer MDA-MB-435 cells with IC 10.59 µM, weaker than the positive control diaminedichloroplatinum with 5.91 μM. Our results suggested that this naphthoquinone could be used as a potential antimicrobial and antitumor agent.
The online version contains supplementary material available at 10.1007/s13205-021-02846-0.
从中国南海沉积物中分离出一种新的深海放线菌12A22，它具有潜在的细胞毒性和抗菌活性。通过对其16S rRNA基因序列进行形态特征研究和系统发育分析，将该放线菌鉴定为[具体名称未给出]。从菌株12A22的发酵液中分离并鉴定出两种化合物，环（-脯氨酸-脯氨酸-酪氨酸-酪氨酸）（[化合物名称未完整给出]）和2-羟乙基-3-甲基-1,4-萘醌（[化合物名称未完整给出]）。化合物[未完整给出的化合物名称]对多种植物病原真菌（[具体真菌名称未完整给出]）和革兰氏阳性菌（[具体细菌名称未完整给出]）表现出显著的抑制活性。特别是，该化合物对[具体真菌名称未完整给出]的抗真菌活性优于阳性对照两性霉素B。此外，化合物[未完整给出的化合物名称]对人乳腺癌MDA-MB-435细胞显示出中等细胞毒性活性，IC50为10.59 μM，比阳性对照二氯二氨铂（5.91 μM）弱。我们的结果表明，这种萘醌可作为一种潜在的抗菌和抗肿瘤剂。
在线版本包含可在10.1007/s13205-021-02846-0获取的补充材料。
