Mohd Zin Nur Bainun, Mohamad Yusof Busyra, Oslan Siti Nurbaya, Wasoh Helmi, Tan Joo Shun, Ariff Arbakariya B, Halim Murni
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
AMB Express. 2017 Dec;7(1):131. doi: 10.1186/s13568-017-0433-y. Epub 2017 Jun 23.
In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs. The lipase was found to be active over a wide range of temperatures and pHs. The activity of lipase can be generally increased by the presence of detergent ingredients such as Tween-80, cetyltrimethylammonium bromide, hydrogen peroxide and phosphate per sulphate. The great compatibility of lipase in commercial detergents has also underlined its potential as an additive ingredient in biodetergent formulations.
近年来，人们致力于探索通过提高产量和利用低成本农业废弃物来降低工业脂肪酶生产成本的方法。椰渣是椰子油和椰奶加工厂的木质纤维素副产品，富含纤维素（36%）和粗脂肪（9%）。一种新分离出的平流层芽孢杆菌已被证明能对椰渣进行纤维素水解，产生可发酵糖。在以酸预处理椰渣进行的深层发酵中，胞外脂肪酶活性最高达到了140 U/mL。发现该脂肪酶在很宽的温度和pH范围内都具有活性。脂肪酶的活性通常可因洗涤剂成分如吐温-80、十六烷基三甲基溴化铵、过氧化氢和过硫酸磷酸盐的存在而提高。脂肪酶在商用洗涤剂中的良好兼容性也凸显了其作为生物洗涤剂配方中添加剂成分的潜力。
