Institute of Microbiology, University of Agriculture , Faisalabad , Pakistan.
Braz J Microbiol. 2012 Apr;43(2):476-81. doi: 10.1590/S1517-83822012000200007. Epub 2012 Jun 1.
The present study was aimed at exploring the native wild isolates of Penicillium chrysogenum series in terms of their penicillin production potential. Apart from the standard medium, the efforts were made to utilize suitable agro-industrial wastes for the maximum yield of penicillin. Two series of P. chrysogenum were isolated from local sources and named as P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2. The native series were found to possess better penicillin production potential than the already reported series of P. chrysogenum. However, P. chrysogenum series UAF R1 was found to be the best candidate for high yield of penicillin starting at 100 hour as compared to P. chrysogenum series UAF R2 which produced the highest yield of penicillin at 150 hours for a shorter period of time. Addition of Corn Steep Liquor (CSL) to the fermentation medium resulted in the production of 1.20g/L penicillin by P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2. The fermentation medium in which Sugar Cane Bagasse (SCB) was replaced with CSL resulted in the highest yield of penicillin (1.92g/L) by both native series of P. chrysogenum. The penicillin production was increased by 62.5% in medium with SCB as compared to that with CSL. The penicillin yield of medium containing lactose and phenyl acetate was higher than that of control medium. Overall results revealed that P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2 may be recommended for better yield of natural penicillin and this efficiency may be further enhanced by utilizing SCB as substrate in the growth medium.
本研究旨在探索青霉素产生菌的原生野生菌株在青霉素生产潜力方面的情况。除了标准培养基外，还努力利用合适的农业工业废物来获得青霉素的最大产量。从当地来源分离出两个青霉素产生菌系列，分别命名为 UAF R1 系列和 UAF R2 系列。与已报道的青霉素产生菌系列相比，发现原生系列具有更好的青霉素生产潜力。然而，与 UAF R2 系列相比，UAF R1 系列在 100 小时时开始就具有更高的青霉素产量，是高产青霉素的最佳候选菌株，而 UAF R2 系列在较短的时间内于 150 小时时产生了最高的青霉素产量。在发酵培养基中添加玉米浆（CSL）可使 UAF R1 系列和 UAF R2 系列产生 1.20g/L 的青霉素。用糖蜜渣（SCB）代替 CSL 的发酵培养基可使两种原生青霉素系列产生最高的青霉素产量（1.92g/L）。与 CSL 相比，在含有 SCB 的培养基中青霉素产量增加了 62.5%。含有乳糖和苯乙酸盐的培养基的青霉素产量高于对照培养基。总体结果表明，UAF R1 系列和 UAF R2 系列的青霉素产生菌可用于提高天然青霉素的产量，并且通过在生长培养基中利用 SCB 作为底物，这种效率可能会进一步提高。
