El-Marsafy M, Abdel-Akher M, El-Saied H
Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1977;132(2):117-22. doi: 10.1016/s0044-4057(77)80053-1.
The conventional penicillin fermentation medium is composed of corn steep liquor, glucose, lactose, minerals, oil, and precursor. The penicillin activity was not affected, due to the addition of carbonate 0-1% or whale oil 0.5% instead of 1%. Also the omission of cupric, magnesium, manganese, zinc sulphates, and acetic acid did not affect the penicillin activity, while the omission of ammonium nitrate and potassium dihydrogen phosphate decreased the penicillin activity in the medium. The penicillin activity of a medium containing 2% calcium superphosphate was higher than that of the control medium, containing 0.4% potassium dihydrogen phosphate and 1% calcium carbonate. Instead of adding the precursor twice, after 0 and 48 hrs, the addition of phenylacetamide in the amount of 0.2%, at the start, did not affect the activity, while addition of phenylacetic acid (0.2%), at the start, decreased the penicillin activity. The omission of the precursors in the medium decreased the penicillin activity measured microbiologically, however, 6-aminopenicillanic acid content was relatively higher as compared with that of the control medium, containing precursor. These results were confirmed, using iodometric assay and paper chromatographic analysis.
传统的青霉素发酵培养基由玉米浆、葡萄糖、乳糖、矿物质、油和前体组成。由于添加了0 - 1%的碳酸盐或0.5%的鲸油（而非1%的鲸油），青霉素活性未受影响。同样，省略硫酸铜、硫酸镁、硫酸锰、硫酸锌和乙酸也不影响青霉素活性，而省略硝酸铵和磷酸二氢钾会降低培养基中的青霉素活性。含有2%过磷酸钙的培养基的青霉素活性高于含有0.4%磷酸二氢钾和1%碳酸钙的对照培养基。在开始时添加0.2%的苯乙酰胺，而非在0小时和48小时后分两次添加前体，不会影响活性，而在开始时添加0.2%的苯乙酸会降低青霉素活性。培养基中省略前体降低了微生物测定的青霉素活性，然而，与含有前体的对照培养基相比，6 - 氨基青霉烷酸含量相对较高。使用碘量法测定和纸色谱分析证实了这些结果。
