Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná , Curitiba, PR , Brasil.
Braz J Microbiol. 2012 Apr;43(2):661-74. doi: 10.1590/S1517-83822012000200031. Epub 2012 Jun 1.
The acidic peatlands of southern Brazil are ecosystems essential for the maintenance of the Atlantic Forest, one of the 25 hot-spots of biodiversity in the world. In this work, we investigated the composition of prokaryotic communities in four histosols of three acidic peatland regions by constructing small-subunit (SSU) rRNA gene libraries and sequencing. SSU rRNA gene sequence analysis showed the prevalence of Acidobacteria (38.8%) and Proteobacteria (27.4%) of the Bacteria domain and Miscellaneous (58%) and Terrestrial (24%) groups of Crenarchaeota of the Archaea domain. As observed in other ecosystems, archaeal communities showed lower richness than bacterial communities. We also found a limited number of Euryarchaeota and of known methanotrophic bacteria in the clone libraries.
巴西南部的酸性泥炭地是维持世界 25 个生物多样性热点之一的大西洋森林的必要生态系统。在这项工作中,我们通过构建小亚基(SSU)rRNA 基因文库和测序,调查了三个酸性泥炭地地区的四个 Histosols 中细菌和古菌群落的组成。SSU rRNA 基因序列分析表明,细菌域的 Acidobacteria(38.8%)和 Proteobacteria(27.4%)以及古菌域的 Miscellaneous(58%)和 Terrestrial(24%)群落占主导地位。与其他生态系统一样,古菌群落的丰富度低于细菌群落。我们还在克隆文库中发现了有限数量的广古菌和已知的甲烷氧化菌。