Heisig P, Kahmann R
Gene. 1986;43(1-2):59-67. doi: 10.1016/0378-1119(86)90008-9.
The mom gene of bacteriophage Mu encodes a DNA modification function. The gene is regulated on the transcriptional level by Dam-specific methylation and a trans-acting Mu function, and on a post-transcriptional level by the product of gene com. The gene encoding the transactivator has been cloned and mapped. By complementation analysis the activation function (also designated Dad) was shown to be the product of gene C. Transactivation of the mom promoter was shown in the following assay: the mom promoter and N-terminal part of com were fused in frame to lacZ. Cells containing such fusion plasmids were infected with M13 clones expressing C in the presence of IPTG and XGal. Successful transactivation results in the formation of blue plaques. Moreover, we have determined the sequence of gene C and found that it has a coding capacity of 140 amino acids. The promoter for C (pc) is likely to be located at least 0.5 kb upstream from the gene. A transcription terminator is found directly downstream from the C-coding region.
噬菌体Mu的mom基因编码一种DNA修饰功能。该基因在转录水平上受Dam特异性甲基化和一种反式作用的Mu功能调控，在转录后水平上受基因com产物的调控。编码反式激活因子的基因已被克隆和定位。通过互补分析表明，激活功能（也称为Dad）是基因C的产物。在以下实验中显示了对mom启动子的反式激活：将mom启动子和com的N端部分与lacZ读框融合。含有此类融合质粒的细胞在IPTG和XGal存在的情况下用表达C的M13克隆进行感染。成功的反式激活导致蓝色噬菌斑的形成。此外，我们确定了基因C的序列，发现它具有编码140个氨基酸的能力。C的启动子（pc）可能位于该基因上游至少0.5 kb处。在C编码区的直接下游发现一个转录终止子。
